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Anti pten rabbit polyclonal antibody

Manufactured by Cell Signaling Technology
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The Anti-PTEN rabbit polyclonal antibody is a laboratory reagent used to detect the PTEN protein in biological samples. PTEN is a tumor suppressor protein that plays a role in cell signaling pathways.

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Lab products found in correlation

Anti gapdh rabbit polyclonal antibody, by Affinity Biosciences (2 mentions) Anti calnexin rabbit polyclonal antibody, by Affinity Biosciences (2 mentions) Anti tsap6 rabbit polyclonal antibody, by Abcam (2 mentions) Anti rab27a sheep polyclonal antibody, by Thermo Fisher Scientific (2 mentions) Rabbit anti gapdh polyclonal antibody, by Proteintech (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Horseradish peroxidase labeled secondary antibody, by Proteintech (1 mentions) Ecl substrate kit, by Merck Group (1 mentions) Phenylmethanesulfonyl fluoride, by Beyotime (1 mentions) Rabbit polyclonal anti akt antibody, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-PTEN (172 citations) Rabbit anti-PTEN (19 citations) Anti-PTEN antibody (18 citations) PTEN antibody (17 citations) Rabbit anti-PTEN antibody (5 citations) Rabbit PTEN antibody (2 citations) Polyclonal rabbit antibody (2 citations)

3 protocols using anti pten rabbit polyclonal antibody

1

Comprehensive Western Blot Analyses

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Electrophoresis of protein samples was performed in 10% SDS-polyacrylamide gels transferred onto a PVDF membrane. The membranes were blocked with 5% nonfat milk in Tris-buffered saline solution (TBS, 150 mM NaCl, 20 mM Tris-HCl, pH = 7.4) and incubated with primary antibodies in TBS, 0.05% Tween 20, overnight at 4 °C. After washing, the blots were reacted with secondary antibodies for 45 minutes and developed using the enhanced chemiluminescence (ECL) detection system. Anti-Calnexin rabbit polyclonal antibody (Cat.#. AF5362), anti-GAPDH rabbit polyclonal antibody (Cat.#. AF7021) were purchased from Affinity Biosciences. Anti-Cytochrome C mouse monoclonal antibody (Cat.#. ab110325), anti-Ascl1 rabbit polyclonal antibody (Cat.#. ab74065), anti-Brn-2 rabbit polyclonal antibody (Cat. #. ab137469), anti-Myt1l rabbit polyclonal antibody (Cat. #. ab139732), anti-CD9 rabbit polyclonal antibody (Cat. #. ab92726), anti-CD63 rabbit polyclonal antibody (Cat. #. ab217345), anti-TSG101 rabbit polyclonal antibody (Cat. #. ab30871), anti-P53 mouse monoclonal antibody (Cat. #. ab26), anti-TSAP6 rabbit polyclonal antibody (Cat. #. ab151566) were purchased from Abcam. Anti-PTEN rabbit polyclonal antibody (Cat.#. 95525) was obtained from Cell Signaling. Anti-Rab27a sheep polyclonal antibody (Cat. #. PA5–47907) was purchased from Thermo Fisher.
Yang Z., Shi J., Xie J., Wang Y., Sun J., Liu T., Zhao Y., Zhao X., Wang X., Ma Y., Malkoc V., Chiang C., Deng W., Chen Y., Fu Y., Kwak K.J., Fan Y., Kang C., Yin C., Rhee J., Bertani P., Otero J., Lu W., Yun K., Lee A.S., Jiang W., Teng L., Kim B.Y, & Lee L.J. (2019). Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation. Nature biomedical engineering, 4(1), 69-83.
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2

Comprehensive Western Blot Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Electrophoresis of protein samples was performed in 10% SDS-polyacrylamide gels transferred onto a PVDF membrane. The membranes were blocked with 5% nonfat milk in Tris-buffered saline solution (TBS, 150 mM NaCl, 20 mM Tris-HCl, pH = 7.4) and incubated with primary antibodies in TBS, 0.05% Tween 20, overnight at 4 °C. After washing, the blots were reacted with secondary antibodies for 45 minutes and developed using the enhanced chemiluminescence (ECL) detection system. Anti-Calnexin rabbit polyclonal antibody (Cat.#. AF5362), anti-GAPDH rabbit polyclonal antibody (Cat.#. AF7021) were purchased from Affinity Biosciences. Anti-Cytochrome C mouse monoclonal antibody (Cat.#. ab110325), anti-Ascl1 rabbit polyclonal antibody (Cat.#. ab74065), anti-Brn-2 rabbit polyclonal antibody (Cat. #. ab137469), anti-Myt1l rabbit polyclonal antibody (Cat. #. ab139732), anti-CD9 rabbit polyclonal antibody (Cat. #. ab92726), anti-CD63 rabbit polyclonal antibody (Cat. #. ab217345), anti-TSG101 rabbit polyclonal antibody (Cat. #. ab30871), anti-P53 mouse monoclonal antibody (Cat. #. ab26), anti-TSAP6 rabbit polyclonal antibody (Cat. #. ab151566) were purchased from Abcam. Anti-PTEN rabbit polyclonal antibody (Cat.#. 95525) was obtained from Cell Signaling. Anti-Rab27a sheep polyclonal antibody (Cat. #. PA5–47907) was purchased from Thermo Fisher.
Yang Z., Shi J., Xie J., Wang Y., Sun J., Liu T., Zhao Y., Zhao X., Wang X., Ma Y., Malkoc V., Chiang C., Deng W., Chen Y., Fu Y., Kwak K.J., Fan Y., Kang C., Yin C., Rhee J., Bertani P., Otero J., Lu W., Yun K., Lee A.S., Jiang W., Teng L., Kim B.Y, & Lee L.J. (2019). Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation. Nature biomedical engineering, 4(1), 69-83.
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3

Western Blot Analysis of Protein Targets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein was extracted using RAPI (Beyotime, Shanghai, People’s Republic of China) buffer mixed with the protease inhibitor phenylmethanesulfonyl fluoride (Beyotime). Equivalent protein was separated by 10% sodium-dodecyl-sulfate-polyacrylamide-gel electrophoresis (Fdbio Science, Hangzhou, People’s Republic of China) and electro-transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA, USA). The membranes were then blocked with 5% bovine serum albumin and incubated with primary antibodies overnight at 4°C, followed by incubation with horseradish-peroxidase-labeled secondary antibody (1: 10 000; Proteintech). Immunodetection was performed using an ECL substrate kit (Millipore). The primary antibodies were: anti-FTCD rabbit polyclonal antibody (1: 5000; Proteintech), anti-PTEN rabbit polyclonal antibody (1: 1000; Cell Signaling Technology, Boston, MA, USA), anti-Akt rabbit polyclonal antibody (1: 1000; Cell Signaling Technology), anti-phospho-Akt-Ser473 (pAkt) rabbit polyclonal antibody (1: 1000; Cell Signaling Technology), anti-PI3K rabbit polyclonal antibody (1: 2000; Cell Signaling Technology), and anti-GAPDH rabbit polyclonal antibody (1: 10 000; Proteintech).
Chen J., Chen Z., Huang Z., Yu H., Li Y, & Huang W. (2019). Formiminotransferase Cyclodeaminase Suppresses Hepatocellular Carcinoma by Modulating Cell Apoptosis, DNA Damage, and Phosphatidylinositol 3-Kinases (PI3K)/Akt Signaling Pathway. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 4474-4484.
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