Rabbit anti dsred abs

Adult runx1^+/+ and runx1^W84X/W84X mutants were incubated in 10 mM bromodeoxyuridine (BrdU, Sigma-Aldrich, St Louis, USA) dissolved in system water for 4 h as described with modification [61 (link)]. Blood smears were obtained as described previously [62 (link)]. Kidney marrow blood cells were fixed by 4% paraformaldehyde, stained with mouse-anti-BrdU (Roche, Basel, Switzerland, cat.#1170376001, 1 : 16) and rabbit-anti-dsRed Abs (Clontech, Mountain View, USA, cat.#632496, 1 : 400), coupled with Alexa Fluor 488 anti-mouse (Abcam, England, cat.#ab150153, 1 : 400) and Alexa 555 anti-rabbit (Abcam, England, cat.#ab150078, 1 : 400) for fluorescent observation. The ratio of rag2:dsRed and BrdU co-staining cells in rag2:dsRed⁺ cells was calculated between runx1^+/+ and runx1^W84X/W84X mutants. TUNEL assay was conducted using In-situ Cell Death Detection Kit (Roche, Basel, Switzerland) as described [63 (link)], coupled with rabbit-anti-dsRed Abs (Clontech, Mountain View, USA, cat.#632496, 1 : 400) and Alexa 555 anti-rabbit (Abcam, England, cat.#ab150078, 1 : 400). The ratio of rag2:dsRed and TUNEL co-staining cells in rag2:dsRed⁺ cells was calculated between runx1^+/+ and runx1^W84X/W84X mutants.