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Anti merlin

Manufactured by Santa Cruz Biotechnology
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Anti-Merlin is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that targets the protein Merlin, also known as Neurofibromin 2 (NF2). Merlin is a tumor suppressor protein involved in regulating cell growth and division. The Anti-Merlin antibody can be used to detect and study the Merlin protein in research applications.

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Merlin (5 citations)

3 protocols using anti merlin

1

Protein Expression Analysis in Cell Lines

Cited 1 time
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Total cell lysates and cytoplasmic and nuclear protein fractions were extracted from cell cultures as described previously [51 (link)]. Standard Western blot analysis of protein expression was carried out using primary anti-HA (rabbit; Cell Signaling Technology), anti-TAZ (rabbit; Cell Signaling Technology), anti-YAP (rabbit; Cell Signaling Technology), anti-E-cadherin (mouse; BD Biosciences), anti-vimentin (rabbit; Cell Signaling Technology), anti-Merlin (rabbit; Santa Cruz Biotechnology), anti-LATS1 (rabbit; Cell Signaling Technology), anti-MST1 (rabbit; Cell Signaling Technology), anti-phosphorylated LATS1 (Thr1079, rabbit; Cell Signaling Technology), anti-phosphorylated MST1 (Thr183)/MST2 (Thr180, rabbit; Cell Signaling Technology), anti-TEAD (rabbit; Cell Signaling Technology), and anti-CTGF (mouse; Santa Cruz Biotechnology) antibodies. Equal protein-sample loading was monitored using an anti- glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody for total cell protein lysates (rabbit; Santa Cruz Biotechnology), an anti-α-tubulin antibody for cytoplasmic fractions (mouse; Oncogene), and an anti-lamin B1 antibody for nuclear fractions (goat; Santa Cruz Biotechnology).
Xie D., Cui J., Xia T., Jia Z., Wang L., Wei W., Zhu A., Gao Y., Xie K, & Quan M. (2015). Hippo transducer TAZ promotes epithelial mesenchymal transition and supports pancreatic cancer progression. Oncotarget, 6(34), 35949-35963.
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2

Immunoblotting Protocol for Cellular Proteins

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Immunoblotting was performed as described previously [22 (link)]. The following primary antibodies were used: anti-merlin (1:500, Santa Cruz Biotechnology, clone A-19), anti-actin (1:2,000, Santa Cruz Biotechnology, clone I-19), anti-RhoA (1:500, Upstate).
Schulz A., Büttner R., Toledo A., Baader S.L., von Maltzahn J., Irintchev A., Bauer R, & Morrison H. (2016). Neuron-Specific Deletion of the Nf2 Tumor Suppressor Impairs Functional Nerve Regeneration. PLoS ONE, 11(7), e0159718.
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3

Protein Expression Analysis Protocol

Cited 1 time
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Total cell lysates, cytoplasmic and nuclear protein fractions were extracted as described previously (20 (link)). Standard Western blotting was carried out using primary anti-Merlin, anti-FOXM1, -β-catenin, -cyclin D1, -c-Myc, -μPAR, -MMP9, and -VEGF (rabbit; Santa Cruz Biotechnology), and anti-MMP2 (rabbit; Cell Signaling Technology) antibodies. Equal protein-sample loading was monitored using an anti-GAPDH antibody for total cell protein lysates (rabbit; Santa Cruz Biotechnology), an anti-α-tubulin antibody for cytoplasmic fractions (mouse; Oncogene), or an anti-histone H1 antibody for nuclear fractions (mouse; Santa Cruz Biotechnology). Secondary antibodies were anti-mouse IgG or anti-rabbit IgG (Santa Cruz Biotechnology). The bands were quantified using the Quantity One analysis software program (version 4.6; Bio-Rad).
Quan M., Cui J., Xia T., Jia Z., Xie D., Wei D., Huang S., Huang Q., Zheng S, & Xie K. (2015). Merlin/NF2 Suppresses Pancreatic Tumor Growth and Metastasis by Attenuating the FOXM1-Mediated Wnt/β-catenin Signaling. Cancer research, 75(22), 4778-4789.
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