After 14 days of the virus injection and fiber implantation, the basic pain behavior test was performed, and the day of establishing the neuropathic pain model was recorded as day 0. CCI was induced as previously described [32 (link)]. Briefly, mice were deeply anesthetized with 1.25–1.5% isoflurane and the right sciatic nerve was exposed at the mid-thigh level. After removal of adherent tissue, three loose ligatures of chromic gut 6/0 (Jinhuan Medical Product Co., Shanghai, China) were tied 1 mm apart around the nerve. Muscle and skin were closed with sutures (Silkam 6/0 and Safil 4/0, respectively; Ethicon). Pain behavior was tested on the third and seventh days after CCI.
Silkam 6 0
Manufactured by Johnson & Johnson
Silkam 6/0 is a surgical suture made of natural silk fibers. It is a non-absorbable, monofilament suture material used for soft tissue approximation and ligation.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using silkam 6 0
1
Chronic Constriction Injury Neuropathic Pain Model
2
Chronic Constriction Injury of Mouse Sciatic Nerve
3
Chronic Constriction Injury Rat Model
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Briefly, rats were deeply anaesthetised with 1.25–1.5% isoflurane and the right sciatic nerve was exposed at the mid-thigh level. After removal of adherent tissue, three loose ligatures with chromic gut 6/0 (SMI) were tied 1 mm apart around the nerve, proximal to the trifurcation (28 (link)). Muscle and skin were closed with sutures (Silkam 6/0 and prolene 4/0, respectively; Ethicon) and wound clips. In control-animals, all steps were identical, except that the sciatic nerve was exposed but not ligated.
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