Anti tf gtx21223

PrP^C-specific antibodies 3F4 and 8H4 were obtained from Signet laboratories (Dedham, MA, USA). 3F4 binds to an epitope on amino acids 109–112 (Human PrP sequence), while 8H4 binds an epitope in the C-terminal amino acids 145–180 of human and mouse PrP^{62 (link)}. Other primary antibodies were obtained from the following sources: anti-ferritin (F5012, Sigma Aldrich, USA), anti-DMT-1 (ab55812, Abcam, USA), anti-TfR (ab84036, Abcam, USA), anti-Tf (GTX21223, GeneTex, USA), and anti-β-actin (MAB1501, Millipore, USA) Secondary antibodies conjugated with HRP were from GE Healthcare (anti-mouse, LNA931V, anti-rabbit, LNA934V). Alexa fluor-conjugated secondary antibodies were from molecular probes, ImmPACT DAB (SK4105) from Vector labs, USA, PNGase F (P0704S) from NEB, USA and Lipofectamine 3000 transfection reagent from Invitrogen, USA. Radiolabeled ⁵⁹FeCl₃ was obtained from Perkin-Elmer, USA. siRNA against PrP (sc36318) and scrambled siRNA (sc37007) were purchased from Santa Cruz Biotechnology Inc, USA. For immunohistochemistry of hamster retinal sections PrP-6C2 (CVI-WUR, Lelystad, Netherland), ferritin (Jackson ImmunoResearch, West Grove, PA, USA), and Iba1 (019–19741, Wako Chemicals, USA) antibodies were used. FAC (F5879) and all other chemicals were from Sigma Aldrich, USA.

Except where specified, all chemicals were obtained from Sigma-Aldrich (St Louis, MO, USA). Protease inhibitor cocktail was from Roche Diagnostics Corp. (Mannheim, Germany). ECL detection reagent was from GE Healthcare (Waukesha, WI, USA). Anti-Ctr1 ab123105, anti-Sod1 ab13499 were from Abcam (Cambridge, UK). Recombinant anti-PrP^C humanized Fab D18 ABR-0D18 was purchased from InPro Biotechnology (South San Francisco, CA, USA). Monoclonal anti-PrP SHA31 A03213 was from BertinPharma (Montigny le Bretonneux, France). Anti-TfR1 13-6800 was from Invitrogen (Paisley, UK). Anti-β-Tubulin III T2200, monoclonal anti-β-Actin Peroxidase (AC-15) A3854, anti-Steap3 AV43515, anti-Atp7a AV33797, anti-human Ferritin F5012 were from Sigma-Aldrich. Anti-Ccs (FL-274) sc-20141, anti-FtH (H-53) sc-25617 were from Santa Cruz Biotechnology (CA, USA). Anti-Cp 611488 was from BD Transduction Laboratories (Milan, Italy). Anti-Tf GTX21223 was from GeneTex (Texas, USA). Anti-Fpn1 NBP1-21502 was from Novus Biologicals (Littleton, CO, USA). The anti-Atp7b N-WNPD#1 was kindly provided by Prof. S. Lutsenko, Johns Hopkins University, Baltimore, MD, USA. O-dianisidine dihydrochloride D3252 was from Sigma-Aldrich. The ELISA kit for Hepdicin SEB979Mu was from USCN (UK).