Anti fancd2

The following primary antibodies were used in this study: anti-Flag (mouse, Sigma F1804), anti-HA [rabbit, Cell Signaling Technology (CST), 3724], anti–COUP-TFII (mouse, R&D Systems, PP-7174-00; rabbit, CST, 6434), anti-TR4 (mouse, R&D Systems, PP-0107B-00), anti-FANCD2 (rabbit, GeneTex, GTX-30142), anti-FANCA (mouse, EMD Millipore, MABC557), anti-FANCI (rabbit, Bethyl Laboratories, A301-254A), anti-MUS81 (mouse, Abcam, ab14387), anti-SLX4 (rabbit, Bethyl Laboratories, A302-270A), anti–p-ATM (Ser¹⁹⁸¹) (mouse, EMD Millipore, 05-740), anti-PCNA (mouse, Santa Cruz Biotechnology, sc-56), anti-POLD3 (rabbit, Bethyl Laboratories, A301-243A), and anti–β-actin (goat, Santa Cruz Biotechnology, sc-1616). The anti-FAN1 antibody was a gift from J. Huang (Zhejiang University, China). The horseradish peroxidase (HRP)–conjugated secondary antibodies (CST) were used for Western blot, and the fluorophore-conjugated secondary antibodies (Invitrogen) were used for indirect immunofluorescence analyses.

Anti-FMRP antibodies used in this study include IgY#C10 (Dury et al., 2013 (link)) and the hybridoma supernatants from mAb1C3 (Devys et al. 1993 (link)). Anti-m⁶A antibody was obtained from Synaptic Systems (Germany). Anti-α-tubulin and anti-GFP are from Abcam, anti-RPA was purchased from Calbiochem, anti-γH2AX and anti-H2AX from Millipore-Sigma, anti-FANCD2 from GeneTex or Novus Biologicals. BLM (Novus Biologicals) and S9.6 antibodies were kindly provided by J-Y. Masson (Laval University). Anti-PICH antibodies were provided by S. Elowe (Laval University) or purchased from Abcam. Anti-H2A, -H3, and -H4 antibodies were from Abcam.