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Anti metap2

Manufactured by Cell Signaling Technology
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Anti-METAP2 is a laboratory reagent used for the detection and study of METAP2 (Methionine Aminopeptidase 2) in biological samples. METAP2 is an enzyme involved in the post-translational processing of proteins. This product can be used in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to investigate the expression and localization of METAP2 in cells and tissues.

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Lab products found in correlation

Anti α tubulin, by Merck Group (2 mentions) Anti yap taz, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti cofilin, by Cell Signaling Technology (1 mentions) Anti phospho cofilin ser3, by Cell Signaling Technology (1 mentions) Anti usp22, by Merck Group (1 mentions) Anti phospho akt ser, by Cell Signaling Technology (1 mentions) Anti pdcd10, by Merck Group (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti phospho yap ser127, by Cell Signaling Technology (1 mentions) Anti egfr, by Cell Signaling Technology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Anti rhoa, by Cell Signaling Technology (1 mentions) Anti phospho egfr, by Cell Signaling Technology (1 mentions) Anti aldoa, by Cell Signaling Technology (1 mentions) Anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Anti hsp90, by Cell Signaling Technology (1 mentions) Anti yap, by Cell Signaling Technology (1 mentions) Coomassie brilliant blue g 250, by Bio-Rad (1 mentions) Western lightning plus ecl, by PerkinElmer (1 mentions)

2 protocols using anti metap2

1

Antibody Validation for Immunoblotting

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The following antibodies were used in this study (with dilution factor for immunoblotting): anti-PKN2 (#2612, 1:1,000), anti-YAP (#14074, 1:1,000), anti-YAP/TAZ (#8418, 1:1,000), anti-phospho-YAP (Ser127) (#4911, 1:1,000), anti-phospho-EGFR (Tyr1068) (#3777, 1:1,000), anti-EGFR (#4267, 1:20,000), anti-phospho-AKT (Ser473) (#4058, 1:1,000), anti-AKT (#9272, 1:5,000), anti-phospho-ERK1/2 (Thr202/Tyr204) (#9101, 1:1,000), anti-ERK1/2 (#9102, 1:5,000), anti-ARIH2/TRIAD1 (#13689, 1:1,000), anti-BTAF1 (#2637, 1:1,000), anti-GNAQ (#14373, 1:1,000), anti-HSP90 (#4877, 1:5,000), anti-ALDOA (#8060, 1:5,000), anti-METAP2 (#12547, 1:1,000), anti-GAPDH (#2118, 1:5,000), anti-RHOA (#2117, 1:1,000), anti-Cofilin (#5175, 1:1,000), anti-phospho-Cofilin (Ser3) (#3313, 1:1,000), from Cell Signaling Technology; anti-α-Tubulin (T6074, 1:20,000) and anti-β-Actin (A1978, 1:20,000) from Sigma; anti-GNB2 (ab81272, 1:1,000), anti-RIC8A (ab97808, 1:1,000), anti-USP22 (ab195289, 1:1,000), anti-CUL5 (ab184177, 1:1,000), anti-RNF7 (ab181986, 1:1,000), anti-PDCD10 (ab180706, 1:1,000), from Abcam; anti-KCTD5 (#15553–1-AP, 1:1,000), anti-PSAT1 (#10501–1-AP, 1:5,000), from Proteintech; Goat Anti-Rabbit IgG Antibody, (H+L) HRP conjugate (#AP307P, 1:5,000), Goat Anti-Mouse IgG Antibody, (H+L) HRP conjugate (#AP308P, 1:5,000), from Millipore Sigma.
Zeng H., Castillo-Cabrera J., Manser M., Lu B., Yang Z., Strande V., Begue D., Zamponi R., Qiu S., Sigoillot F., Wang Q., Lindeman A., Reece-Hoyes J.S., Russ C., Bonenfant D., Jiang X., Wang Y, & Cong F. (2019). Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC. eLife, 8, e50223.
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2

Western Blot Analysis of MetAP2 Protein

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We performed western blot analysis as previously described (32 (link)–35 (link)). Cells were cultured in a 60-mm dish and lysed in lysis buffer [50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1% (w/v) sodium dodecyl sulfate, 1% (v/v) Triton X-100, 1% (w/v) sodium deoxycholate, and 1 mM phenylmethylsulfonyl fluoride] at 4°C with sonication. The cell lysates were centrifuged at 15,000 × g for 10 min to eliminate debris. Protein concentrations were measured by Coomassie Brilliant Blue G-250 staining (Bio-Rad Laboratories), and loading buffer [350 mM Tris-HCl, pH 6.8, 30% (w/v) glycerol, 0.012% (w/v) bromophenol blue, 6% (w/v) SDS and 30% (v/v) 2-mercaptoethanol] was added to each lysate. After electrophoresis, proteins (15 µg/lane) were transferred to polyvinylidene fluoride membranes and immunoblotted with anti-MetAP2 (#12547S; Cell Signaling Technology, Inc.) or anti-α-tubulin (#T5168; Merck KGaA). Signals were detected by ECL using Western Lightning Plus-ECL (PerkinElmer, Inc.) or Immobilon Western Chemiluminescent HRP substrate (Merck KGaA).
Shimizu S., Kawahara R, & Simizu S. (2021). Methionine aminopeptidase-2 is a pivotal regulator of vasculogenic mimicry. Oncology Reports, 47(2), 31.
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