Gastric cancer cells (AGS, MNK45) were transfected with lentiviral vectors expressing shRNA-p53 or scrambled sequence control shRNA (GenePharma, Suzhou, China) for 3 days and selected with puromycin (sigma, Shanghai, China) for 2 weeks.
Scrambled sequence control shrna
Manufactured by GenePharma
Sourced in China
Scrambled sequence control shRNA is a laboratory tool used to assess the effects of short hairpin RNA (shRNA) knockdown experiments. It contains a randomly generated shRNA sequence that does not target any known gene. This control shRNA is designed to help evaluate the specificity of observed phenotypes associated with gene silencing by shRNA.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using scrambled sequence control shrna
1
Silencing p53 in Gastric Cancer
2
Stable Transfection of TPC-1 Cells
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The DePTC cells (TPC-1) were stably transfected with lentiviral vectors expressing shRNA-p53 corresponding to p53 nucleotides 611-629 [52 (link)] or scrambled sequence control shRNA (GenePharma, 20 Suzhou, China) for 3 days and then selected with 1.0 μg/ml puromycin (sigma, Shanghai, China) for 2 weeks.
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