The mouse blood was collected via a cardiac puncture in microtainer K2E (BD, Cat # 365974, Franklin Lakes, NJ, USA) and centrifuged at 1600× g for 15 min at 4 °C to measure the plasma 17β-estradiol levels. The supernatant plasma was collected, and the levels of 17β-estradiol were assessed using the 17 beta Estradiol ELISA kit (Abcam, Cat # ab108667, Cambridge, UK) following the manufacturer’s protocol. To measure the IL-1β release from the GT1b-stimulated primary mixed glia, we treated ATP 30 min prior to supernatants collection to induce the secretion of IL-1β, and collected supernatants of mixed glia centrifuged at 500× g for 5 min to discard the cell debris. The IL-1β levels were measured using the mouse IL-1 beta Quantikine ELISA kit (R&D Systems Inc., Cat # MLB00C, Minneapolis, MN, USA) following the manufacturer’s protocol.
Microtainer k2e
Manufactured by BD
Sourced in United States
The Microtainer K2E is a collection tube designed for the collection and transportation of small sample volumes. It contains K2EDTA as an anticoagulant to prevent blood clotting.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using microtainer k2e
1
Quantification of Plasma Estradiol and IL-1β
2
Plasma Triglyceride Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Blood was collected in EDTA-coated tubes (Microtainer K2E, BD) and centrifuged (3500 rpm, 15 min) to separate plasma. Livers were flash frozen in liquid nitrogen, then stored at −80 °C until homogenization in isopropanol. Triglyceride levels were measured using reagents from Wako Diagnostics per manufacturer's protocol.
3
Lipid Quantification in Murine Tissues
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