Hcx pl apo 40 0.85 corr cs objective

Manufactured by Leica

The HCX PL APO 40×/0.85 CORR CS objective is a high-quality laboratory microscope objective produced by Leica. It has a magnification of 40x and a numerical aperture of 0.85. The objective is designed for use in a variety of microscopy applications.

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Lab products found in correlation

Tcs sp5 confocal microscope, by Leica (3 mentions) Pl apo 63 0.40 cs objective, by Leica (2 mentions) Hc pl apo 10 0.40 cs objective, by Leica (1 mentions)

Spelling variants of this product

HCX PL APO (50 citations) HCX PL APO CS (36 citations) HCX PL APO ×40 (10 citations)

3 protocols using hcx pl apo 40 0.85 corr cs objective

Imaging Starved and Refed Caenorhabditis elegans

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Animals were mounted on 5% agar with 10 mM NaN₃. Soon after immobilization, images were captured using a Leica HCX PL APO 40×/0.85 CORR CS objective or an HC PL APO 10×/0.40 CS objective on a Leica TCS-SP5 confocal microscope. For examination of effects of starvation and refeeding (Figure 6A,B and Figure 6—figure supplement 1B,C), animals that were starved for two hours in basal buffer (50 mM NaCl, 5 mM potassium phosphate [pH 6.0], 1 mM CaCl₂, 1 mM MgSO₄, 0.05% gelatin) or refed on fresh HB101-seeded NGM plates again for the indicated times after the 2 hr starvation were observed.

Ohno H., Yoshida M., Sato T., Kato J., Miyazato M., Kojima M., Ida T, & Iino Y. (2017). Luqin-like RYamide peptides regulate food-evoked responses in C. elegans. eLife, 6, e28877.

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Visualization and Measurement of ASER Cilia

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Day one adults were mounted on 5% agar and anesthetized by 10 mM NaN₃, or 100 μM levamisole. Images were captured using a Leica HCX PL APO 40×/0.85 CORR CS objective or an HC PL APO 63×/0.40 CS objective on a Leica TCS-SP5 confocal microscope. The length of ASER sensory cilium was measured by simple neurite tracer plugin of ImageJ. All depicted representative fluorescence images were Z-stacked.

Park C., Sakurai Y., Sato H., Kanda S., Iino Y, & Kunitomo H. (2021). Roles of the ClC chloride channel CLH-1 in food-associated salt chemotaxis behavior of C. elegans. eLife, 10, e55701.

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Imaging and Measuring ASER Cilium

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Day 1 adults were mounted on 5% agar and anesthetized by 10 mM NaN3, or 100 μm levamisole. Images were captured using a Leica HCX PL APO 40×/0.85 CORR CS objective or an HC PL APO 63×/0.40 CS objective on a Leica TCS-SP5 confocal microscope. The length of ASER sensory cilium was measured by simple neurite tracer plugin of ImageJ. All depicted representative fluorescent images were Z-stacked.

Park C., Sakurai Y., Sato H., Kanda S., Iino Y., & Kunitomo H. (2020). Roles of the ClC Chloride Channel CLH-1 in Food-associated Salt Chemotaxis Behavior ofC. elegans.

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