Total RNA was extracted from cells and tissues by Trizol reagent (Invitrogen, Shanghai, China) and GeneJET RNA Purification Kit (Invitrogen), respectively. RT SuperMix Reverse Transcription Kit (Vazyme Biotech, Nanjing, China) was employed for reverse transcription from RNA into cDNA, followed by qRT-PCR using SYBR Premix Ex Taq II Kit (Vazyme Biotech). The relative expression was calculated by the 2−ΔΔCT method, GAPDH was utilized to normalize the relative expression levels of circRNA and linear mRNA, and U6 was utilized to normalize the expression level of microRNA. The primer sequences were listed in Supplementary Table S1 .
Rt supermix reverse transcription kit
Manufactured by Vazyme
Sourced in China
RT SuperMix Reverse Transcription Kit is a reagent kit designed for the reverse transcription of RNA into cDNA. It contains all the necessary components, including reverse transcriptase, RNase inhibitor, and buffer, to efficiently convert RNA into cDNA for further downstream applications such as PCR or qPCR.
Automatically generated - may contain errors
2 protocols using rt supermix reverse transcription kit
1
Quantitative Analysis of RNA Expression
2
Comprehensive RNA Extraction and Analysis
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The intracellular total RNA was extracted using TRIzol reagent (Invitrogen), and the tissue sample total RNA was extracted using the GeneJET RNA Purification Kit (Invitrogen) in accordance with the reagent instructions. The RT SuperMix reverse transcription kit (Vazyme Biotech) was used to reverse transcribe RNA into cDNA, and the SYBR Premix Ex Taq II (Vazyme Biotech) was used for qRT‐PCR. The relative gene expression level was calculated according to the 2−ΔΔCT method, GAPDH was used to normalize the relative expression levels of circRNA and linear mRNA, whereas U6 was utilized to normalize the miRNA expression level. All primers and the related sequences used in the experiment are listed in Table 1 .
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