Rabbit anti fads2

Cells in the logarithmic growth phase were seeded at a density of 5 ×10⁶ cells/well in a 6-well plate and incubated for 48 h. The cells were collected and lysed with radio-immunoprecipitation assay (RIPA) lysis buffer supplemented with protease and phosphatase inhibitors on ice for 30 min. The samples were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE; 10% SDS), and the gel was transferred onto polyvinylidene difluoride (PVDF) membranes for western blot analysis. The following antibodies were used for the detection of proteins: rabbit anti-FADS2 (1:1000, Abcam) and mouse anti-HMGCR (1:1000, Abcam. Mouse anti-GAPDH (1:5000, Abcam) was used as a loading control. Proteins were visualised using anti-mouse or anti-rabbit HRP-conjugated secondary antibodies (1:5000, Zhongshan Jinqiao biotechnology company) and ECL-Plus (Millipore). The resulting bands were analysed and quantified using the ImageJ^® 1.49 g software (National Institutes of Health, Bethesda, MD, United States). Each experiment was repeated in triplicates.