Polyclonal bak

Manufactured by Santa Cruz Biotechnology

Sourced in Canada

Polyclonal Bak is an antibody product developed by Santa Cruz Biotechnology. It is a collection of antibodies that recognize the Bak protein, which is involved in the regulation of apoptosis, or programmed cell death. The core function of Polyclonal Bak is to provide researchers with a tool to study the Bak protein and its role in various biological processes.

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2 protocols using polyclonal bak

Antibody-Based Protein Expression Analysis

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The following antibodies were used: the primary antibodies for cleaved PARP-1 (cleaved p25), DR5, and Smac/DIABLO were purchased from GeneTex whereas AIF was purchased from Thermo Scientific. Antibodies against antibody against polyclonal caspase-3, monoclonal DR4, monoclonal p21, polyclonal p27, monoclonal Cdk2, polyclonal Cdk4, polyclonal cyclin D1, polyclonal cyclin E, polyclonal cIAP-1, polyclonal c-FLIPL, monoclonal survivin, polyclonal Bak, polyclonal Bax, monoclonal Bcl-xL, polyclonal Bid, polyclonal GADD153, monoclonal p-ERK, polyclonal ERK2 and polyclonal Akt1 were purchased from Santa Cruz. Antibodies against monoclonal caspase-8, monoclonal cytochrome c, monoclonal COX IV, polyclonal p-Rb, monoclonal Rb, polyclonal XIAP, monoclonal p-p38, polyclonal p38, polyclonal p-JNK (Thr183/Tyr185), monoclonal polyclonal JNK, p-Akt (Ser473) and β-actin were purchased from Cell Signaling Technology. The horseradish peroxidase (HRP)-labeled anti-mouse and anti-rabbit secondary antibodies were purchased from Santa Cruz. Bradford reagent was purchased from Bio-rad. Mitochondrial/cytosol fractionation kit was purchased from BioVision.

Phang C.W., Karsani S.A., Sethi G, & Abd Malek S.N. (2016). Flavokawain C Inhibits Cell Cycle and Promotes Apoptosis, Associated with Endoplasmic Reticulum Stress and Regulation of MAPKs and Akt Signaling Pathways in HCT 116 Human Colon Carcinoma Cells. PLoS ONE, 11(2), e0148775.

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Immunoprecipitation and Immunoblotting Assay

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Immunoprecipitation and immunoblotting were performed as described (26 ). Primary antibodies employed were: Polyclonal BAX, BCL-2, and MCL-1 (PharMingen; San Diego, CA). Cleaved Poly(ADP-ribose) Polymerase PARP, cleaved caspase-3, ERK1/2, phospho-AKT (Ser473), phospho-p70S6K, and BIM (Cell Signaling Technology; Beverly, MA). AIF, cytochrome c, polyclonal BAK, BCL-X_L, AKT, and p70S6K (Santa Cruz Biotechnology, Santa Cruz, CA). α-tubulin (Calbiochem).

Rahmani M., Nkwocha J., Hawkins E., Pei X., Parker R.E., Kmieciak M., Leverson J.D., Sampath D., Ferreira-Gonzalez A, & Grant S. (2018). Co-targeting BCL-2 and PI3K induces BAX-dependent mitochondrial apoptosis in AML cells. Cancer research, 78(11), 3075-3086.

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