CLL cells were stained for ZAP-70 protein expression as previously described [8 (link)]. Briefly, ZAP-70-positive cells were evaluated in the PB via analysis of the surface expression of CD19 and CD5 antigens, as well as intracellular expression of ZAP-70 by flow cytometry. The percentage of CD19+CD5+ZAP-70+ cells was determined. MoAbs used for analyses included anti-ZAP-70 PE (Clone 1E7.2, IgG1, BD Biosciences, Franklin Lakes, NJ, USA) and anti-CD19 FITC (Clone HIB19, IgG1) and anti-CD5 PE-Cy5 (Clone UCHT2, IgG1) (BD Pharmingen, San Diego, CA, USA). The cutoff point for ZAP-70 positivity in leukemic cells was ≥20 %.
Anti zap 70 pe
Manufactured by BD
Sourced in United States
Anti-ZAP-70 PE is a fluorochrome-conjugated antibody that binds to the intracellular protein ZAP-70. ZAP-70 is a tyrosine kinase that plays a critical role in T-cell receptor signaling. This antibody can be used to detect and analyze ZAP-70 expression in various cell types, particularly in T cells, using flow cytometry.
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2 protocols using anti zap 70 pe
1
Evaluation of ZAP-70 Expression in CLL
2
CLL Immunophenotyping and FISH Analysis
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CLL cells were stained for CD38 antigen and ZAP-70 protein expression (as described previously [28] (link)). Fresh PB samples were stained with FITC mouse anti-human CD19 (Clone SJ25C1), PE-Cy5 mouse anti-human CD5 (Clone UCHT2) and CD38 FITC (Clone HIT2) or anti-ZAP-70 PE (Clone 1E7.2) (BD Biosciences). A cut-off point for ZAP-70 positivity in leukemic cells was ≥ 20%. Patients with CD38 expression lower or higher than 30% were classified as CD38 negative or positive, respectively.
I-FISH analysis. Detection of del(17p13.1) and del(11q22.3) was a part of the routine diagnostic practice. We used a previously described method [29] (link).
I-FISH analysis. Detection of del(17p13.1) and del(11q22.3) was a part of the routine diagnostic practice. We used a previously described method [29] (link).
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