Apoptosis was assessed by annexin V-fluorescence isothiocyanate (FITC)/PI apoptosis detection kit (BD biosciences, USA) according to the manufacturer’s instructions. Simvastatin-treated BCa cells were harvested, washed twice with cold PBS and centrifuged at 200 g for 10 min, resuspended in 100 ml 1x binding buffer, incubating with FITC- annexin V and PI for 15 min at room temperature in the dark. 400 μl of 1x binding buffer was added to each tube and assessed on flow cytometry.
Annexin 5 fluorescence isothiocyanate fitc pi apoptosis detection kit
Manufactured by BD
Sourced in United States
The Annexin V-fluorescence isothiocyanate (FITC)/PI apoptosis detection kit is a laboratory tool used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a dye that stains DNA, to distinguish between viable, early apoptotic, and late apoptotic/necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using annexin 5 fluorescence isothiocyanate fitc pi apoptosis detection kit
1
Quantifying Simvastatin-Induced Apoptosis
2
Cell Cycle and Apoptosis Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For cell cycle analysis, 1 × 106 cells were harvested and fixed in 70% ice cold ethanol at –20ºC overnight. After centrifugation, pellets were resuspended with PBS containing 50 μg/ml propidium iodide (Sigma-Aldrich, USA) and 0.1 mg/ml RNaseA (20 μg/ml in PBS) in the dark. After incubation at 37ºC for 30 min, the DNA content distribution was analyzed by flow cytometry analysis (Beckman, Cat. #FC500). Cell apoptosis was analyzed by flow cytometry analysis using Annexin V-fluorescence isothiocyanate (FITC)/PI apoptosis detection kit (BD Biosciences, San Jose, CA, USA), according to the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!