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Ab181013

Manufactured by Abcam
Sourced in United States

Ab181013 is a monoclonal antibody that recognizes the Vimentin protein. Vimentin is a type III intermediate filament protein that is expressed in various cell types, including mesenchymal cells. This antibody can be used for the detection of Vimentin in a range of applications.

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2 protocols using ab181013

1

Evaluation of Chemokine Signaling Pathway

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The following reagents and materials were used: TAC and monobenzone (Cat. No. 1642802 and 1445506, respectively; Sigma-Aldrich, China), specific antibodies against chemokine (C-X-C motif) ligand CXCL9, CXCL10, CXCR3 and HSP70 (Cat. No. ab202961, ab271208, ab181013 and ab181606, respectively; Abcam, Cambridge, MA, USA), anti-CD8 monoclonal antibody (Cat. No. 55397; Cell Signaling Technology, Danvers, MA, USA), Trizol and cDNA synthesis kits (Cat. No. 15596018 and 4374967, respectively; Invitrogen, Carlsbad, CA, USA), primers for reverse transcription–quantitative polymerase chain reaction ([RT-qPCR]; Invitrogen, Shanghai, China) and specific antibodies against p-STAT3, STAT3 and GAPDH (Cat. No. 9145 T, 91395 and 5174S, respectively; Cell Signaling Technology).
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2

Osteoclast Differentiation Protocol

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Recombinant soluble murine M-CSF (315-02), RANKL (315-11), and CXCL10 (250-16) were purchased from PeproTech (Rocky Hill, NJ, USA). Primary antibodies against mouse P65 (8242), p-P65 Ser536 (3033), IκB-α (4814), and p-IκB-α Ser32 (2859) used in western blots were obtained from Cell Signaling Technology (Beverly, MA, USA). Rabbit polyclonal antibody against CXCR3 (ab181013) was purchased from Abcam (Cambridge, MA, USA). Mouse antibody against β-actin (BM5180) was from Boster (Wuhan, China).
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