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2 protocols using anti p yap1 4911s antibody

1

Characterization of Mammary Cell Lines

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We purchased mammary epithelial cell line MCF10A and breast cancer cell lines MDA-MB-231, HCC-1806, MDA-MB-468, BT549, MCF7, T47D, AU565, Hs578T, BT474, HCC-1824 from ATCC. All the cell lines have tested and confirmed by the Mayo Clinic medical genome facility Center. USP9X WT and its inactive mutants were purchased from MRC PPU (University of Dundee). The luciferase reporter constructs were kindly provided by Kunliang Guan 9 (link). YAP1 cDNA was kindly provided by Junjie Chen 11 (link) and we subcloned it to pCMV-HA and pLV.3-FLAG vectors.
Anti-USP9X (Catalog No: 55054-1-AP) antibody was purchased from Proteintech. Anti-p-YAP1 (4911S) antibody was purchased from cell signaling. Anti-YAP1 (SC15407) and anti-Ub (sc-8017) antibodies were purchased from Santa Cruz. Antibodies against HA (H9658), FLAG (F1804), β-actin (A1978) and β-Tubulin (T8328) were purchased from sigma.
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2

Characterization of Mammary Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
We purchased mammary epithelial cell line MCF10A and breast cancer cell lines MDA-MB-231, HCC-1806, MDA-MB-468, BT549, MCF7, T47D, AU565, Hs578T, BT474, HCC-1824 from ATCC. All the cell lines have tested and confirmed by the Mayo Clinic medical genome facility Center. USP9X WT and its inactive mutants were purchased from MRC PPU (University of Dundee). The luciferase reporter constructs were kindly provided by Kunliang Guan 9 (link). YAP1 cDNA was kindly provided by Junjie Chen 11 (link) and we subcloned it to pCMV-HA and pLV.3-FLAG vectors.
Anti-USP9X (Catalog No: 55054-1-AP) antibody was purchased from Proteintech. Anti-p-YAP1 (4911S) antibody was purchased from cell signaling. Anti-YAP1 (SC15407) and anti-Ub (sc-8017) antibodies were purchased from Santa Cruz. Antibodies against HA (H9658), FLAG (F1804), β-actin (A1978) and β-Tubulin (T8328) were purchased from sigma.
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