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Vcam 1 pe

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VCAM-1-PE is a fluorescent-labeled antibody that binds to the Vascular Cell Adhesion Molecule-1 (VCAM-1) protein. It is used for the detection and analysis of VCAM-1 expression in flow cytometry applications.

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Lab products found in correlation

Cd45 pe cy7, by BD (2 mentions) Cd31 pe cy7, by BD (2 mentions) Sca 1 apc cy7, by BD (2 mentions) Fibroblast growth factor 2 (fgf2), by R&D Systems (2 mentions) Collagenase type 2, by Thermo Fisher Scientific (1 mentions) Horse serum, by Thermo Fisher Scientific (1 mentions) Dispase, by Thermo Fisher Scientific (1 mentions) 40 μm cell strainer, by BD (1 mentions) Cd31 fitc, by BioLegend (1 mentions) F10 medium, by Thermo Fisher Scientific (1 mentions) Facsaria 2, by BD (1 mentions) Sca1 apc, by BioLegend (1 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions) Collagenase type 2, by Worthington (1 mentions) Cd45 fitc, by BioLegend (1 mentions)

Spelling variants of this product

VCAM-1 (42 citations) PE anti-mouse CD106/VCAM-1 (2 citations) Anti-human CD106 (VCAM-1) PE (2 citations) PE)-anti-VCAM-1 (2 citations)

3 protocols using vcam 1 pe

1

Isolation of Highly Purified Myogenic Cells

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To obtain highly purified myogenic cells, mononucleated cells were isolated from uninjured muscles. Cells in sorting medium (10% HS, in Ham’s F-10) were incubated with VCAM-1-PE (Invitrogen), integrin-α7–649 (AbLab), CD31-PE/Cy7 (BD Biosciences), CD45-PE/Cy7 (BD Biosciences), and Sca1-APC/Cy7 (BD Biosciences) antibodies. Propidium iodide (PI) was used for viable cell gating. Myogenic cells had the following profile: VCAM1+/ integrin-α7+/CD31-/CD45-/Sca1-/PI-. For culture and derivation of myoblasts, myogenic cells were plated onto 1:1000 ECM (Extra Cellular Matrix) coated plates in Growth media (20% FBS, 5 ng/ml FGF-2 (R&D systems) in Ham’s F10).
Chen Y., Ikeda K., Yoneshiro T., Scaramozza A., Tajima K., Wang Q., Kim K., Shinoda K., Sponton C.H., Brown Z., Brack A, & Kajimura S. (2018). Thermal stress induces glycolytic beige fat formation via a myogenic state. Nature, 565(7738), 180-185.
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2

Isolation of Highly Purified Myogenic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
To obtain highly purified myogenic cells, mononucleated cells were isolated from uninjured muscles. Cells in sorting medium (10% HS, in Ham’s F-10) were incubated with VCAM-1-PE (Invitrogen), integrin-α7–649 (AbLab), CD31-PE/Cy7 (BD Biosciences), CD45-PE/Cy7 (BD Biosciences), and Sca1-APC/Cy7 (BD Biosciences) antibodies. Propidium iodide (PI) was used for viable cell gating. Myogenic cells had the following profile: VCAM1+/ integrin-α7+/CD31-/CD45-/Sca1-/PI-. For culture and derivation of myoblasts, myogenic cells were plated onto 1:1000 ECM (Extra Cellular Matrix) coated plates in Growth media (20% FBS, 5 ng/ml FGF-2 (R&D systems) in Ham’s F10).
Chen Y., Ikeda K., Yoneshiro T., Scaramozza A., Tajima K., Wang Q., Kim K., Shinoda K., Sponton C.H., Brown Z., Brack A, & Kajimura S. (2018). Thermal stress induces glycolytic beige fat formation via a myogenic state. Nature, 565(7738), 180-185.
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3

Isolation of Muscle Stem Cells

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Isolation of mononuclear myogenic cells from hindlimb muscles was performed as described (Liu et al., 2015 (link)) with minor modifications. Briefly, muscles were chopped in cold PBS and digested using 800 U/mL collagenase type 2 (Worthington, cat# LS004177) in F10 medium (Gibco, cat# 11550-043) supplemented with horse serum (Gibco, cat# 26050-088) and antibiotic–antimycotic (Gibco, cat# 15240-062) for 60 min followed by another digestion using 1000 U/mL collagenase type 2 and 11 U/mL dispase (Gibco, cat# 17105-041) for 30 min. The supernatants were filtered through a 40 μm cell strainer (Falcon, cat# 352340) and centrifuged to yield a cell suspension. The following antibodies were used: CD31-FITC (BioLegend, cat# 102406; RRID: AB_312901; 1/100 dilution), CD45-FITC (BioLegend, cat# 103108, RRID: AB_312973; 1/100 dilution), Sca1-APC (BioLegend, cat# 122512, RRID: AB_756197; 1/100 dilution), and Vcam1-PE (Invitrogen, cat# 12-1061-82, RRID: AB_2572573; 1/100 dilution). Cells were isolated using the FACS Aria II (Becton, Dickinson and Company) and analyzed by FlowJo v10.1r7 (BD Life Sciences).
Sakai H., Sawada Y., Tokunaga N., Tanaka K., Nakagawa S., Sakakibara I., Ono Y., Fukada S.I., Ohkawa Y., Kikugawa T., Saika T, & Imai Y. (2022). Uhrf1 governs the proliferation and differentiation of muscle satellite cells. iScience, 25(3), 103928.
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