MCF-7 and SGC7901 cells were seeded into 96-well plates at a density of 5 × 103 cells/well for 24 h. The cells were incubated with different concentrations of DTP (0, 0.35, 0.7, and 1.4 μM) for 24 h followed by photoirradiation with an energy density of 14 J/cm2 for 14 min or 16 J/cm2 for 17 min at a wavelength of 650 nm. The cells were also incubated in the presence of 12 μM P38 inhibitor SB203580 (Beyotime, China), 12 μM JNK inhibitor SP600125 (Beyotime, China), 12 μM ERK inhibitor FR180204 (Beyotime), or 10 mM ROS inhibitor N-acetyl-L-cysteine (NAC; Beyotime). Each experimental group included four wells per group. Eight hours later, the cells were treated with 10 µL of Cell Counting Kit-8 (CCK-8; Beyotime) for 2 h at 37°C. Absorbance was measured at 450 nm using a microplate reader. The cell survival rate was calculated using the following formula: Cell survival rate (%) = ([ODexperimental − ODblank]/[ODcontrol − ODblank]) × 100.
Ros inhibitor n acetyl l cysteine nac
Manufactured by Beyotime
Sourced in China
N-acetyl-L-cysteine (NAC) is a synthetic compound that functions as a reactive oxygen species (ROS) inhibitor. It is a precursor to the antioxidant glutathione and can help regulate cellular redox states.
Automatically generated - may contain errors
Lab products found in correlation
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
P38 inhibitor sb203580, by Beyotime (1 mentions)
Jnk inhibitor sp600125, by Beyotime (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
Hoechst staining kit, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (1 mentions)
Annexin 5 fitc 7 aad apoptosis detection kit, by BD (1 mentions)
Rapamycin, by Meilun (1 mentions)
2 protocols using ros inhibitor n acetyl l cysteine nac
1
Photodynamic Therapy Efficacy in Cancer Cells
2
Cinobufagin's Anti-Angiogenic Effects
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Cinobufagin was purchased from Chenguang Biotechnology (Baoji, China). MTT and DMSO were purchased from Sigma‐Aldrich (St Louis, MO, USA). RPMI 1640, FBS, and antibiotics were purchased from Thermo Fisher Scientific (Boston, MA, USA). HUVEC was purchased from ATCC (Manassas, VI, America). Mice hemangioendothelioma cell EOMA was purchased from JNObio (Guangzhou, China). Hoechst staining kit, JC‐1 kit for mitochondrial membrane potential, reactive oxygen species (ROS) assay kit and ROS inhibitor N‐acetyl‐l ‐cysteine (NAC) were purchased from Beyotime Biotechnology (Shanghai, China). Annexin V‐FITC/7AAD apoptosis detection kit was purchased from Becton Dickinson (BD, Franklin Lakes, NJ, USA). Antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA) and Abcam (Cambridge, UK). Rapamycin was purchased from Meilunbio (Dalian, China). Endostatin was purchased from Simcere (Shandong, China).
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