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Goat anti human igg cross adsorbed secondary

Manufactured by Thermo Fisher Scientific

Goat anti-human IgG cross-adsorbed secondary is a secondary antibody used in various immunoassay techniques. It is generated by immunizing goats with human IgG and is then cross-adsorbed to remove cross-reactivity with other species. This secondary antibody can be used to detect and quantify human IgG in samples.

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2 protocols using goat anti human igg cross adsorbed secondary

1

Immunofluorescence Imaging of Cultured Cells

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For immunofluorescence experiments, HKs were cultured directly on glass slides (Ibidi) until confluent, as described above. Live cells were labeled with monoclonal antibodies at a concentration of 10 μg/ml for 30 min at 4°C. Cells were then fixed on ice using 3.7% paraformaldehyde for 10 min followed by permeabilization in 0.1% triton for 10 min at room temperature. Goat anti-human IgG cross-adsorbed secondary (Life Technologies) was then used prior to mounting with prolong gold containing Dapi (Life Technologies). Widefield images were acquired using a DMRXA2 microscope (Leica, Wetzler, Germany) equipped with a 63X/1.40 NA oil immersion objective. Images were acquired with an ORCA digital camera (Hamamatsu Photonics, Bridgewater, NJ). Analysis was preformed using Fiji ImageJ.
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2

Immunofluorescence Imaging of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunofluorescence experiments, HKs were cultured directly on glass slides (Ibidi) until confluent, as described above. Live cells were labeled with monoclonal antibodies at a concentration of 10 μg/ml for 30 min at 4°C. Cells were then fixed on ice using 3.7% paraformaldehyde for 10 min followed by permeabilization in 0.1% triton for 10 min at room temperature. Goat anti-human IgG cross-adsorbed secondary (Life Technologies) was then used prior to mounting with prolong gold containing Dapi (Life Technologies). Widefield images were acquired using a DMRXA2 microscope (Leica, Wetzler, Germany) equipped with a 63X/1.40 NA oil immersion objective. Images were acquired with an ORCA digital camera (Hamamatsu Photonics, Bridgewater, NJ). Analysis was preformed using Fiji ImageJ.
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