Vitamin supplement

Manufactured by Merck Group

The Vitamin Supplement is a laboratory product designed to provide essential vitamins and nutrients for research and development purposes. It is a concentrated formulation containing a range of vitamins, minerals, and other beneficial compounds. The core function of this product is to supply the necessary nutritional components required for various scientific applications and experiments.

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Lab products found in correlation

L cysteine, by Merck Group (2 mentions) D fructose, by Merck Group (2 mentions) D maltose, by Merck Group (2 mentions) Sterile filtered fetal bovine serum, by Merck Group (2 mentions) D cellobiose, by Merck Group (2 mentions) Trace mineral supplement, by Merck Group (2 mentions) Powerwave ht microplate spectrophotometer, by Agilent Technologies (2 mentions) Soft sided vinyl chamber, by Coy Laboratory Products (2 mentions) D2438, by Merck Group (2 mentions)

2 protocols using vitamin supplement

Anaerobic Growth of Ruminococcus gnavus

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We grew R. gnavus ATCC 29149 in BHI medium (37 g/L) containing: 5% sterile-filtered fetal bovine serum (Sigma-Aldrich), 1% vitamin K1-hemin solution (BD Biosciene), 1% trace mineral supplement (ATCC), 1% vitamin supplement (ATCC), 1 g/L D-(+)-cellobiose (Sigma-Aldrich), 1 g/L D-(+)-maltose (Sigma-Aldrich), 1 g/L D-(+)-fructose (Sigma-Aldrich) and 0.5 g/L L-cysteine (Sigma-Aldrich). Growth occurred under anaerobic conditions (atmosphere 5% H2, 20% CO2, 75% N2) in a soft-sided vinyl chamber (Coy Laboratory Products, Michigan, USA). We sterilized the media using a Corning filter unit (0.22 µm pore diameter). All metabolite standards (Sigma-Aldrich) were brought to 100 mM in DMSO (Sigma-Aldrich, D2438) prior to dilution for dose assays. Overnight bacterial cultures were diluted 100-fold in appropriate media and 40 µL were dispensed per well in 384-well plates (low evaporation lid, Costar 3680) containing metabolites or DMSO control. The plates were shaken to ensure homogeneity and bacterial growth was monitored anaerobically (absorbance at 600 nm) in a microplate reader (PowerWave HT Microplate Spectrophotometer, BioTek) for 24 hours at 37°C without shaking. Values recorded for DMSO controls and metabolite-treated triplicates were averaged.

Franzosa E.A., Sirota-Madi A., Avila-Pacheco J., Fornelos N., Haiser H.J., Reinker S., Vatanen T., Brantley Hall A., Mallick H., McIver L.J., Sauk J.S., Wilson R.G., Stevens B.W., Scott J.M., Pierce K., Deik A.A., Bullock K., Imhann F., Porter J., Zhernakova A., Fu J., Weersma R.K., Wijmenga C., Clish C.B., Vlamakis H., Huttenhower C, & Xavier R.J. (2018). Gut microbiome structure and metabolic activity in inflammatory bowel disease. Nature microbiology, 4(2), 293-305.

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Anaerobic Growth of Ruminococcus gnavus

Check if the same lab product or an alternative is used in the 5 most similar protocols

We grew R. gnavus ATCC 29149 in BHI medium (37 g/L) containing: 5% sterile-filtered fetal bovine serum (Sigma-Aldrich), 1% vitamin K1-hemin solution (BD Biosciene), 1% trace mineral supplement (ATCC), 1% vitamin supplement (ATCC), 1 g/L D-(+)-cellobiose (Sigma-Aldrich), 1 g/L D-(+)-maltose (Sigma-Aldrich), 1 g/L D-(+)-fructose (Sigma-Aldrich) and 0.5 g/L L-cysteine (Sigma-Aldrich). Growth occurred under anaerobic conditions (atmosphere 5% H2, 20% CO2, 75% N2) in a soft-sided vinyl chamber (Coy Laboratory Products, Michigan, USA). We sterilized the media using a Corning filter unit (0.22 μm pore diameter). All metabolite standards (Sigma-Aldrich) were brought to 100 mM in DMSO (Sigma-Aldrich, D2438) prior to dilution for dose assays. Overnight bacterial cultures were diluted 100-fold in appropriate media and 40 μL were dispensed per well in 384-well plates (low evaporation lid, Costar 3680) containing metabolites or DMSO control. The plates were shaken to ensure homogeneity and bacterial growth was monitored anaerobically (absorbance at 600 nm) in a microplate reader (PowerWave HT Microplate Spectrophotometer, BioTek) for 24 hours at 37°C without shaking. Values recorded for DMSO controls and metabolite-treated triplicates were averaged.

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