Antibodies against total p38

Antibodies against total p38, phospho-p38, total p65, and phospho-p65 were purchased from Cell Signaling (Danvers, MA). Anti-TLR2 antibodies used in Flow Cytometry analysis were purchased from Biolegend (San Diego, CA). S-[2,3-Bis(palmitoyloxy)-(2-RS)-propyl]- N-palmitoyl-(R)-Cys-Ser-Lys₄-OH (Pam3Cys) was purchased from InVivogen (San Diego, CA). Purified, mammalian-expressed murine and human recombinant IFN-β (rIFN-β) was purchased from PBL (Piscataway, NJ).

Antibodies against total p38, phospho-p38, total p-38, phospho-ERK1/2, total ERK 1/2, total p65, phospho p65, and total IL-1β were purchased from Cell Signaling (Danvers, MA). Anti-IL-10 and anti-IL-27 neutralizing antibodies were purchased from e-Bioscience (San Diego, CA). All antibodies used in Flow Cytometry were purchased from Biolegend (San Diego, CA). Recombinant murine IL-10 and IL-27 was purchased from Biolegend. Protein-free phenol/water extracted Escherichia-coli K235 LPS was prepared as described elsewhere (23 (link)). S-[2,3-Bis(palmitoyloxy)-(2-RS)-propyl]-N-palmitoyl-(R)-Cys-Ser-Lys₄-OH (Pam3Cys) was purchased from InVivogen (San Diego, CA). Purified mammalian-expressed recombinant IFN-β was purchased from PBL (Piscataway NJ). Recombinant murine IFN-γ was kindly provided by Genentech, Inc. (San Francisco, CA). Necrostatin was purchased from Santa Cruz (Santa Cruz, CA). L18-MDP and C12-iE-DAP were purchased from Invivogen (San Diego, CA).