Alexa fluor 568 goat anti mouse igg

Manufactured by Abcam

Sourced in United Kingdom

Alexa Fluor 568 goat anti-mouse IgG is a secondary antibody conjugated with the Alexa Fluor 568 fluorescent dye. It is designed to detect and visualize mouse immunoglobulin G (IgG) in various applications such as immunohistochemistry, flow cytometry, and Western blotting.

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Lab products found in correlation

Lsm 710, by Zeiss (2 mentions) Mab377, by Merck Group (2 mentions) Alexa fluor 488 goat anti rabbit igg, by Abcam (2 mentions) Triton x 100, by Merck Group (1 mentions) Paraformaldehyde pfa, by Merck Group (1 mentions) Cd31 antibody, by Abcam (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Qtracker 525, by Thermo Fisher Scientific (1 mentions) Ab52894, by Abcam (1 mentions) Ab180904, by Abcam (1 mentions) Ab150077, by Abcam (1 mentions) Alexa fluor 488 goat anti rat igg, by Abcam (1 mentions)

Spelling variants of this product

Alexa Fluor 568 (42 citations) Alexa 568 (7 citations) Goat Anti-Mouse IgG H&L-Alexa Fluor® 568 (6 citations) Anti-IgG mouse (2 citations) Mouse anti (2 citations)

4 protocols using alexa fluor 568 goat anti mouse igg

Labeling and Immunostaining of GBM Cells

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Primary GBM cells were labelled using Qtracker 525 (ThermoFisher Scientific, UK). Cells were subcultured at a density of 2

\times

^{4}

cells per well of a 48-well plate and incubated overnight. Then 200

μ

L of 15 nM Qtracker diluted in SFM was added per well. Cells were then incubated for 6 h before washing them twice with SFM.
Tumoroids were immunofluorescently stained with CD31 antibody. Gel constructs were fixed using 4% paraformaldehyde (PFA, Sigma-Aldrich, UK) for 1 hour followed by washes with phosphate-buffered saline (PBS, Sigma-Aldrich, UK). The constructs were subsequently permeabilised with 0.25% Triton X-100 (Sigma-Aldrich, UK) and blocked with 1% bovine serum albumin (BSA, Sigma-Aldrich, UK). Cells were incubated overnight with CD31 antibody (Abcam, UK) diluted at 1:100 followed by 2 h of incubation with 1:500 AlexaFluor 568 goat anti-mouse IgG (abcam, UK) diluted at 1:500. Washes with PBS followed both incubation periods.

Tatla A.S., Justin A.W., Watts C, & Markaki A.E. (2021). A vascularized tumoroid model for human glioblastoma angiogenesis. Scientific Reports, 11, 19550.

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Immunohistochemical Analysis of EGFR Expression

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To assess EGFR protein expression in the PVN, rats were deeply anesthetized with urethane (1.5 g/kg, IP) and transcardially perfused with 4% paraformaldehyde. Brains were removed, post-fixed in 4% paraformaldehyde for 24 h at 4°C and then cryoprotected in 30% sucrose for 48 h at 4°C. Brains were frozen in OCT compound on dry ice and cut into 20 μm coronal sections using a cryostat. EGFR immunoreactivity was detected using a rabbit anti-rat primary antibody to EGFR (1:250, Catalog No: ab52894, Abcam, Cambridge, MA). Neurons were identified using a mouse anti-rat primary antibody to NeuN (1:200, Catalog No: MAB377, Millipore, Billerica, MA). Immunoreactivity for EGFR and NeuN was visualized using secondary antibodies Alexa fluor 488 goat anti-rabbit IgG (1:200, Catalog No: ab150077, Abcam, Cambridge, MA) and Alexa fluor 568 goat anti-mouse IgG (1:200, Catalog No: ab 175702, Abcam, Cambridge, MA), respectively.
To verify the transfection potential of the siRNAs, as indicated by visualization of GFP in the PVN, rats were sacrificed without perfusion and brains were removed quickly. Brains were immediately frozen in liquid nitrogen and then cut into 20 μm coronal sections using a cryostat.
Immunofluorescence for EGFR and NeuN and GFP fluorescence were visualized using a confocal laser-scanning microscope (Zeiss LSM 710, Carl Zeiss, Inc, Oberkochen, Germany).

Yu Y., Wei S.G., Weiss R.M, & Felder R.B. (2021). Silencing Epidermal Growth Factor Receptor in Hypothalamic Paraventricular Nucleus Reduces ERK1/2 Signaling and Sympathetic Excitation in Heart Failure Rats. Neuroscience, 463, 227-237.

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Immunofluorescence Analysis of IL-17RA in Rat PVN

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To determine IL-17RA expression in the PVN, rats were transcardially perfused with 4% paraformaldehyde. Brains were removed, post-fixed in 4% paraformaldehyde for 24 h at 4°C and then cryoprotected in 30% sucrose for 48 h at 4°C. Brains were sectioned into 20 μm coronal sections using a cryostat. The sections were incubated with a rabbit anti-rat primary antibody to IL-17RA (ab180904, 1:50, Abcam, Cambridge, MA) and a mouse anti-rat primary antibody to NeuN (MAB377, 1:200, Millipore, Billerica, MA), followed by secondary antibodies Alexa fluor 488 goat anti-rabbit IgG (ab150077, 1:200, Abcam, Cambridge, MA) and Alexa fluor 568 goat anti-mouse IgG (ab 175702, 1:200, Abcam), respectively. To verify the transfection potential of the siRNAs by visualization of GFP in the PVN, rats were sacrificed without perfusion and brains were removed quickly. Brains were immediately frozen in liquid nitrogen and then sectioned into 20 μm coronal sections using a cryostat. Immunofluorescence for IL-17RA and NeuN and GFP were visualized by a confocal laser-scanning microscope (Zeiss LSM 710, Carl Zeiss, Inc., Oberkochen, Germany).

Yu Y., Weiss R.M, & Wei S.G. (2022). Brain Interleukin-17A contributes to neuroinflammation and cardiac dysfunction in rats with myocardial infarction. Frontiers in Neuroscience, 16, 1032434.

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ACE2 Protein Expression in HEK293T Cells

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HEK293T-hACE2 cells were cultured on the microscope cover glass in 24-well plates at the density of 1x10⁵ cells/well. After 24 h, cells were washed in PBS then fixed in 4% PBS-buffered formalin for 30 min, then washed with PBS and incubated with anti-human ACE2 antibody or S^RBD-mFc and 2xS^RBD-mFc proteins. Afterward, cells were stained with secondary antibodies including: Alexa Fluor 488 Goat anti-Rat IgG (Abcam, ab175473) or Alexa Fluor 568 Goat anti-mouse IgG (Abcam, ab175473) at 1:1000 dilution. After staining, cells were examined using a laser scanning confocal microscopy (Zeiss 880).

Liu Z., Yang C., Zhang H., Cao G., Wang S., Yin S, & Wang Y. (2022). SARS-CoV-2 tetrameric RBD protein blocks viral infection and induces potent neutralizing antibody response. Frontiers in Immunology, 13, 960094.

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