Ultrasensitive colorimetric assay for nitric oxide synthase kit

Phospho-PKCzeta (T410) and phospho-eNOS (S1177), and eNOS antibodies, were purchased from Cell Signaling (Danvers, MA). VEG and actin antibodies and protein A/G plus for immunoprecipitation experiments were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The PKCzeta antibody and 3-nitrotyrosine ELISA were purchased from Abcam (Cambridge, MA). An Ultrasensitive Colorimetric Assay for Nitric Oxide Synthase kit was bought from Oxford Biomedical Research (Oxford, MI). PRKCzeta recombinant human protein was purchased from Life Technologies (Carlsbad, CA). Endothelial bovine nitric oxide synthase and N-acetyl cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO). The SuperFect transfection reagent was bought from Qiagen (Valencia, CA). Horseradish peroxidase (HRP) conjugated secondary anti-mouse and anti-rabbit antibodies were purchased from Promega (Madison, WI). Enhanced chemiluminescence for immunoblot development and signal detection was purchased from Amersham Biosciences (Piscataway, NJ). IGFBP-3 NB plasmid DNA was a gift from Dr. Maria B. Grant (University of Indiana).