The following primary antibodies were used in this study: mouse αVE–cadherin/CD144–Alexa Fluor 647 (BD), rabbit αErk1/2 (Santa Cruz), mouse αpErk1/2 (Santa Cruz), rabbit αpMyosin LC2 Thr18/Ser19 (Cell Signaling), rabbit αpPAK1/2/3 Ser141 (Invitrogen), rabbit αvinculin (Sigma), rabbit αGAPDH (Cell Signaling), mouse αHA (Sigma), rabbit αLAMP1 (Cell Signaling), rabbit αKCTD10 (Sigma), rabbit αPaxillin Tyr31 (Sigma), mouse αRac1 (BD), rabbit αRhoC (Cell Signaling), rabbit αRhoA (Cell Signaling), rabbit αRhoB (Santa Cruz), mouse αRhoB (Santa Cruz), mouse αUbiquitin (FK-2; Boston Biochem), rabbit αUbiquitin, Lys63-specific (Apu3; Millipore), rabbit αCullin-3 (Cell Signaling), mouse αCullin-2 (BD), mouse αCullin-1 (Santa Cruz), rabbit αKCTD10 (Sigma), rat αmouseCD29 (9eg7; BD), rabbit αIntegrin α-5 (Abcam), and rabbit p-NF-κB p65 (Ser536; Cell Signaling). Mouse anti–HA agarose was purchased from Sigma.
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