Enzyme-linked immunosorbent assay (ELISA) (Boehringer), Spectrometers (Shimadzu), microplate 96-well plate, and microplate reader, centrifuge, and incubator (Memmert).
Spectrometers
Manufactured by Shimadzu
Spectrometers are scientific instruments used to analyze the composition and properties of materials by measuring the interaction between the material and electromagnetic radiation. They detect and quantify the absorption, emission, or reflection of light by a sample across different wavelengths of the electromagnetic spectrum.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using spectrometers
1
Quantitative Immunoassay using ELISA
2
Isolation and Estrogenic Evaluation of Phenolphthalein from Momordica charantia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The compound after fractionation by HPLC is yielded a white amorphous powder (98.89% purity) further subjected to spectral studies. The IR spectrum was obtained on 460 Shimadzu spectrometers. 1 H NMR spectra were recorded on JEOL JNM -A 500 NMR spectrometer and solvent methanol was used for spectral analysis. Mass spectra were recorded on JMS HX -110 with a data system and on JMS -DA 500 mass spectrometers.
Phytoestrogenic nature of phenolphthalein: Bioassay-guided fractionation led to the isolation of amorphous phenolphthalein compound. Hence, it was subjected to in vivo estrogenic activity (Animal ethics Reg. No. 34800/2001/CPAEA). Colony bred immature female albino rats of 25-30 days old, weighing between 30-40g were bilaterally ovariectomised to observe the phytoestrogenic nature of phenolphthalein. The treatments were given from 7 days. On day 8 th the rat uteri were dissected out and weighed for gravimetric analysis. Vaginal smears changes were recorded daily from these experimental immature rats.
The Figure 1 show a schematic diagram showing the preparation and isolation of phytoestrogen (Phenolphthalein) from Momordica charantia Seeds
Phytoestrogenic nature of phenolphthalein: Bioassay-guided fractionation led to the isolation of amorphous phenolphthalein compound. Hence, it was subjected to in vivo estrogenic activity (Animal ethics Reg. No. 34800/2001/CPAEA). Colony bred immature female albino rats of 25-30 days old, weighing between 30-40g were bilaterally ovariectomised to observe the phytoestrogenic nature of phenolphthalein. The treatments were given from 7 days. On day 8 th the rat uteri were dissected out and weighed for gravimetric analysis. Vaginal smears changes were recorded daily from these experimental immature rats.
The Figure 1 show a schematic diagram showing the preparation and isolation of phytoestrogen (Phenolphthalein) from Momordica charantia Seeds
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!