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Tead1 clone 610923

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TEAD1 clone 610923 is a laboratory equipment product offered by BD. It is a DNA clone that encodes the TEAD1 (TEA Domain Family Member 1) gene. TEAD1 is a transcription factor that plays a role in cellular processes. The product is intended for use in research applications, but a detailed description of its function or intended use is not available.

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Lab products found in correlation

Total polyclonal, by Cell Signaling Technology (2 mentions) β actin clone a5441, by Merck Group (2 mentions) Collagen 1 clone col 1, by Abcam (2 mentions) Ezh2 polyclonal, by BD (2 mentions) Total histone h3 polyclonal, by Cell Signaling Technology (2 mentions) Tri methyl histone h3 lys27 polyclonal, by Cell Signaling Technology (2 mentions) Phospho smad3 polyclonal, by Cell Signaling Technology (2 mentions) Pu 1 polyclonal, by Cell Signaling Technology (2 mentions) Hrp conjugated secondary antibody, by Agilent Technologies (2 mentions) Bradford protein assay, by Bio-Rad (2 mentions)

Spelling variants of this product

TEAD1 (7 citations)

2 protocols using tead1 clone 610923

1

Quantitative Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein concentrations were determined using the Bradford Protein Assay according to the manufacturer’s protocol (Bio-Rad). Same amounts of protein were loaded on a Tris-Glycine buffered gel. Proteins were separated by SDS-PAGE and transferred to PVDF membrane. The membrane was incubated with the appropriate primary antibody and HRP-conjugated secondary antibodies (Dako, Glostrup, Denmark). The following antibodies were used: β-Actin clone A5441 (1/10000, Sigma-Aldrich), Collagen I clone COL-1 (1/1000, abcam), TEAD1 clone 610923 (1/500, BD Biosciences), EZH2 polyclonal (4905, 1/2000), total histone H3 polyclonal (9715, 1/1000), Tri-Methyl-Histone H3(Lys27) polyclonal (9733, 1/1000), total polyclonal (9513, 1/1000) or phospho-Smad3 polyclonal (9520, 1/1000) and PU.1 polyclonal (2266, 1/500) (all Cell Signaling). As secondary antibodies in western blot anti-mouse polyclonal (P0447, 1/1500) or anti-rabbit polyclonal (P0448, 1/2000) HRP-conjugated secondary antibodies (all Dako) were used. Blots were visualized using enhanced chemiluminescence (ECL). β-Actin was used as a loading control. Western Blots were quantified using ImageJ Software (version 1.46r).
Wohlfahrt T., Rauber S., Uebe S., Luber M., Soare A., Ekici A., Weber S., Matei A.E., Chen C.W., Maier C., Karouzakis E., Kiener H.P., Pachera E., Dees C., Beyer C., Daniel C., Gelse K., Kremer A.E., Naschberger E., Stürzl M., Butter F., Sticherling M., Finotto S., Kreuter A., Kaplan M.H., Jüngel A., Gay S., Nutt S.L., Boykin D.W., Poon G.M., Distler O., Schett G., Distler J.H, & Ramming A. (2019). SPI1/PU.1 controls fibroblast polarization and tissue fibrosis. Nature, 566(7744), 344-349.
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2

Quantitative Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein concentrations were determined using the Bradford Protein Assay according to the manufacturer’s protocol (Bio-Rad). Same amounts of protein were loaded on a Tris-Glycine buffered gel. Proteins were separated by SDS-PAGE and transferred to PVDF membrane. The membrane was incubated with the appropriate primary antibody and HRP-conjugated secondary antibodies (Dako, Glostrup, Denmark). The following antibodies were used: β-Actin clone A5441 (1/10000, Sigma-Aldrich), Collagen I clone COL-1 (1/1000, abcam), TEAD1 clone 610923 (1/500, BD Biosciences), EZH2 polyclonal (4905, 1/2000), total histone H3 polyclonal (9715, 1/1000), Tri-Methyl-Histone H3(Lys27) polyclonal (9733, 1/1000), total polyclonal (9513, 1/1000) or phospho-Smad3 polyclonal (9520, 1/1000) and PU.1 polyclonal (2266, 1/500) (all Cell Signaling). As secondary antibodies in western blot anti-mouse polyclonal (P0447, 1/1500) or anti-rabbit polyclonal (P0448, 1/2000) HRP-conjugated secondary antibodies (all Dako) were used. Blots were visualized using enhanced chemiluminescence (ECL). β-Actin was used as a loading control. Western Blots were quantified using ImageJ Software (version 1.46r).
Wohlfahrt T., Rauber S., Uebe S., Luber M., Soare A., Ekici A., Weber S., Matei A.E., Chen C.W., Maier C., Karouzakis E., Kiener H.P., Pachera E., Dees C., Beyer C., Daniel C., Gelse K., Kremer A.E., Naschberger E., Stürzl M., Butter F., Sticherling M., Finotto S., Kreuter A., Kaplan M.H., Jüngel A., Gay S., Nutt S.L., Boykin D.W., Poon G.M., Distler O., Schett G., Distler J.H, & Ramming A. (2019). SPI1/PU.1 controls fibroblast polarization and tissue fibrosis. Nature, 566(7744), 344-349.
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