Chat ires cre mice 129s6 chattm2 cre lowl j

The mGluR2−/− mouse line (B6;129S-Grm2^tm1Nak/NakRbrc) (Moreno et al., 2011 (link)) was a generous gift from Dr. Javier González-Maeso at Virginia Commonwealth University. Chat-IRES-Cre mice (129S6-Chat^tm2(cre)Lowl/J) and floxed tdTomato mice (129S6-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/J) were acquired from the Jackson Laboratory. Drd4–GFP mice, in which posterior-preferring DSGCs are selectively labeled, were originally developed by MMRRC in the Swiss Webster background, and were subsequently backcrossed to C57BL/6 background. All strains were backcrossed to the C57BL/6 background in our laboratory, and crossed to each other to create the lines used in this study.
All experiments were performed on healthy mice of normal immune status which had not been previously used for tests or procedures. Mice were housed in 12 hr-12 hr light-dark cycles in groups of 2–5 per cage and were provided with food and water ad libitum. For electrophysiology experiments, mice of ages P21–P40 of either sex were used. All procedures to maintain and use mice were in accordance with the University of Chicago Institutional Animal Care and Use Committee (Protocol number ACUP 72247) and in conformance with the NIH Guide for the Care and Use of Laboratory Animals and the Public Health Service Policy.