Alexa fluor 488 affinipure donkey anti chicken igy

Antibodies were from the following sources: rat anti-Yl and rabbit anti-Yolk antibodies were from Dr. Mahowald [48 (link)]. mouse anti-Rab7 (1:10, Developmental Studies Hybridoma Bank (DSHB)), mouse anti-Rab11 (1:200, BD Transduction Laboratories (#610657)), mouse anti-actin (1:10000, MAB1501, Chemicon, and #ab-6276, Abcam); mouse anti-αTubulin (1:10000, DM1A, Sigma); mouse anti-HA (12CA5, Roche); chicken anti-GFP (Aves. 1:10,000 for Western blot and 1:2,000 for immunofluorescent staining). Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (1:1000, 703-545-155) and Rhodamine Red AffiniPure Donkey Anti-Mouse IgG (1:1000, 715-295-151) from Jackson ImmunoResearch Inc. TRITC-Phalloidin (Phalloidin–Tetramethylrhodamine B isothiocyanate) (1:200, P1951, Sigma). Alexa Fluor 488-Phalloidin (1:200, A-12379), Alexa Fluor 594-Phalloidin (1:200, A-12381), Alexa Fluor 647-Phalloidin (1:200, A-30107), Alexa Fluor 680-(A-21076) were from Molecular Probes (Invitrogen). Alexa Fluor 680-(A-21076) and Alexa Fluor 800-(926–32212) conjugated secondary antibodies for immunoblotting (1:10,000) were from Molecular Probes (Invitrogen) and LI-COR, respectively.

Primary antibodies used in this study for immunofluorescence are the following: rabbit anti-vasa (1:5000; R. Lehmann), guinea pig anti–phospho–myosin II (1:1000; R. Ward), chicken anti-vasa (1:500; R. Lehmann), and rabbit anti-RhoGEF2 (1:2500; J. Grosshans).
Secondary antibodies used in this study for immunofluorescence are the following: Cy3 AffiniPure Donkey Anti-Rabbit IgG (immunoglobulin G) (Jackson ImmunoResearch, 711-165-152), Cy3 AffiniPure Donkey Anti-Guinea Pig IgG (Jackson ImmunoResearch, 706-165-148), Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG (Jackson ImmunoResearch, 711-545-152), and Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (Jackson ImmunoResearch, 703-545-155).
Primary antibodies used for immunoblotting are the following: mouse anti-mNeongreen (1:1000; Chromotek, 32F6), rabbit anti-mNeongreen (1:1000; Cell Signaling Technology, 53061), mouse anti–α-tubulin (1:4000; Sigma-Aldrich, T6199), mouse anti-RFP (1:1000; Chromotek, 6G6), rabbit anti-GST (1:2000; Cell Signaling Technology, 2622), rabbit anti–phospho-AMPK (1:1000; Cell Signaling Technology, 2535), and mouse anti-AMPK (1:3000; Bio-Rad, MCA2672GA).
Secondary antibodies used in this study for immunoblotting are the following: horseradish peroxidase (HRP) goat anti-rabbit IgG (1:10,000; Abcam, ab6721) and HRP rabbit anti-mouse IgG (1:10,000; Abcam, ab6728).