Dioscin was obtained from Sigma‐Aldrich (SMB00576, USA). We fully dissolved Dioscin in 0.5% sodium carboxymethyl cellulose (CMC‐Na, IS9000, Solarbio) solution in distilled water for in vivo experiments, and we fully dissolved Dioscin in 0.01% dimethyl sulfoxide (DMSO, D8371, Solarbio) for in vitro experiments.23
Dioscin
Manufactured by Solarbio
Sourced in China
Dioscin is a naturally occurring saponin compound extracted from various plant sources. It functions as a surfactant, reducing the surface tension of liquids. Dioscin is commonly used in biochemical and cell biology applications as a membrane permeabilizing agent.
Automatically generated - may contain errors
Lab products found in correlation
Menadione, by Merck Group (1 mentions)
Candida glabrata atcc2001, by China General Microbiological Culture Collection Center (1 mentions)
Candida parapsilosis atcc22019, by China General Microbiological Culture Collection Center (1 mentions)
C albicans atcc10231, by China General Microbiological Culture Collection Center (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Methanoic acid, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Dioscin, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Histone h3, by Proteintech (1 mentions)
4 protocols using dioscin
1
Preparation and Dissolution of Dioscin for In Vivo and In Vitro Studies
2
Antifungal Activity Evaluation of Dioscin
Check if the same lab product or an alternative is used in the 5 most similar protocols
C. albicans SC5314, C. albicans ATCC10231, Candida glabrata ATCC2001, and Candida parapsilosis ATCC22019 were obtained from China General Microbiological Culture Collection Center (CGMCC). All these strains were maintained on YPD (yeast extract 1%, peptone 2%, dextrose 2%) medium and stored at -80°C. Before each test, a colony from fungal strains subcultured twice on YPD agar was transferred into YPD medium and incubated overnight at 30°C with a speed of 140 rpm for propagation.
Dioscin was bought from Solarbio Life Sciences. N-acetyl-D-glucosamine (GlcNAc), XTT, MTT, menadione, propidium iodide (PI), and RPMI-1640 medium were bought from Sigma.
+ Expand
C. albicans SC5314, C. albicans ATCC10231, Candida glabrata ATCC2001, and Candida parapsilosis ATCC22019 were obtained from China General Microbiological Culture Collection Center (CGMCC). All these strains were maintained on YPD (yeast extract 1%, peptone 2%, dextrose 2%) medium and stored at -80°C. Before each test, a colony from fungal strains subcultured twice on YPD agar was transferred into YPD medium and incubated overnight at 30°C with a speed of 140 rpm for propagation.
Dioscin was bought from Solarbio Life Sciences. N-acetyl-D-glucosamine (GlcNAc), XTT, MTT, menadione, propidium iodide (PI), and RPMI-1640 medium were bought from Sigma.
3
Quantitative Analysis of Dioscin in M. charantia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The standard of dioscin and 1-butly-3-methylimidazolium tetrafluoroborate ([C4MIM]BF4, ≥97.0%, CAS: 174501-65-6) were purchased from Solarbio (Beijing, China). Vanillin (≥99.0%, CAS: 121-33-5) was obtained from Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). Chromatographic grade methanoic acid (≥98.0%, CAS: 64-18-6) and acetonitrile (≥99.9%, CAS: 75-05-8) were obtained from Sigma-Aldrich-Fluka (Shanghai, China) and used for high performance liquid chromatography (HPLC) analysis. Other kinds of solvent used in the experiment, such as ethanol, were analytical grade and purchased from Sichuan Xilong Chemical Works (Chengdu, China). M. charantia powder (M. charantia pericarp and inner tissue were dehydrated, then vacuum dried) was purchased from Hunan Changsha Bojian Biological Technology Co., Ltd. (Changsha, China).
4
Dioscin-induced Apoptosis Regulation in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dioscin (purity ≥98%) was purchased from Beijing Solarbio Science & Technology Co., Ltd. Cells were treated with different concentrations of Dioscin (0, 0.5, 1 or 2 µM) for 24 or 48 h, or with 1 µM Dioscin for different durations (0, 2, 4 or 8 h). Nuclear Protein Extraction kit (cat. no. R0050) was purchased from Beijing Solarbio Science & Technology Co., Ltd and was used to extract nuclear proteins prior to western blotting. Primary antibodies targeted against GAPDH (cat. no. 60004-1-Ig) and histone H3 (cat. no. 17168-1-AP) were obtained from ProteinTech Group, Inc. Primary antibodies targeted against LATS1 (cat. no. 3477), p-LATS1 (Ser909; cat. no. 9157), p-MST (cat. no. 49332), MST2 (cat. no. 3952), YAP (cat. no. 4912), p-YAP (Ser397; cat. no. 13619), cyclin D1 (cat. no. 92G2) and BAX (cat. no. 5023) were purchased from Cell Signaling Technologies, Inc. The primary antibody targeted against BCL-2 (cat. no. WL01556) was purchased from Wanleibio Co., Ltd. The primary antibody targeted against RASSF1A (cat. no. ab91212) was purchased from Abcam. The goat anti-mouse or anti-rabbit HRP-conjugated secondary antibody (cat. nos. S0002 or S0001) was obtained from Affinity Biosciences.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!