Total RNA was extracted using FavorPrep™ Total RNA Kit (Favorgen, Taiwan) according to the manufacturer's protocol. Then, the quantity and quality of mRNA were confirmed by Nanodrop (ODs = 260 m) and gel electrophoresis (1.5%), respectively. At the reverse transcription step, complementary DNA (cDNA) was synthesized using a first strand cDNA synthesis BioFact™ RT‐Kit (BioFACT, Daejeon, Korea) and analysed by polymerase chain reaction (PCR). We quantified the mRNA expression by quantitative PCR (qPCR) using the 2−ΔΔCT relative quantitation method and normalized by the expression level of GAPDH as the internal control. The primers are listed in Table 1 .
Favorprep total rna kit
Manufactured by Favorgen Biotech
The FavorPrep™ Total RNA Kit is a laboratory equipment product designed for the extraction and purification of total RNA from various biological samples. It utilizes a silica-membrane-based technology to efficiently capture and purify RNA molecules.
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2 protocols using favorprep total rna kit
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Quantitative Real-Time PCR Protocol
2
Quantification of Gene Expression by RT-qPCR
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Total RNA was isolated with use of the FavorPrep total RNA kit (Favorgen Biotech, Taiwan) according to the manufacturer protocol. cDNA was synthesized, subsequent to DNase treatment and used for real‐time quantitative PCR (RT‐qPCR) with use of the MyiQ Single‐colour Real‐Time Detection System (Bio‐Rad laboratories, Hercules, USA) for quantification with Sybr Green. All primers used in this study were obtained from Biolegio (Nijmegen, The Netherlands). See Table S1 for primer sequences. Gene expression levels were normalized to the expression of the human RPLP0 gene and the relative expression levels of all genes of interest were measured using the 2−ΔΔCT method.[21 (link)
] The RNA sequencing data in this study were generated previously by members from our group[17 (link)
] and can be found at the GEO database (GSE98483 ).
] The RNA sequencing data in this study were generated previously by members from our group[17 (link)
] and can be found at the GEO database (
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