Log In Sign up
The largest database of trusted experimental protocols

Cd3 fitc cd8 pe cd4 apc

Manufactured by BD
Visit Supplier
Sourced in United States

The CD3-FITC/CD8-PE/CD4-APC is a multi-color flow cytometry reagent that simultaneously detects the expression of CD3, CD8, and CD4 on cells. This reagent allows for the identification and quantification of T cell subsets.

Automatically generated - may contain errors

Lab products found in correlation

Cd3 apc, by BD (1 mentions) Cd4 percp, by BD (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Cytomics fc500, by Beckman Coulter (1 mentions)

Close spelling variants of this product

CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC BD Multitest™ CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC reagent CD3-PE/FITC/APC CD4- PE/FITC/APC CD8-APC/FITC CD8-APC CD3-APC CD4-APC CD8-FITC CD8-PE

2 protocols using cd3 fitc cd8 pe cd4 apc

1

Multicolor Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The main reagents and equipment are (1) monoclonal antibodies: CD3/CD4/CD8/CD45 four-color antibody (product number: 561707), CD3APC (product number: 30062), CD4PerCP (product number: 100538), CD3-FITC/CD8-PE/CD4-APC (product number: 561644), and γδTCR APC (product number: 561995) (produced by Becton Dickinson, USA); (2) FACSCalibur flow cytometer (produced by Becton Dickinson, USA); (3) EDTA-K2 anticoagulant vacuum blood collection tube (produced by Becton Dickinson, USA); (4) microplate reader (RT-6100, produced by Rayto, USA); and (5) centrifuge (produced by Eppendorf, Germany, type 5424R).
Zhao N., Zhang T., Zhao Y., Zhang J, & Wang K. (2021). CD3+T, CD4+T, CD8+T, and CD4+T/CD8+T Ratio and Quantity of γδT Cells in Peripheral Blood of HIV-Infected/AIDS Patients and Its Clinical Significance. Computational and Mathematical Methods in Medicine, 2021, 8746264.
+ Open protocol
+ Expand
2

Peripheral Blood T Lymphocyte Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood was collected from the patients prior to treatment for BC. Flow cytometry was used to determine T lymphocyte subset ratios in peripheral blood (21 (link)). For the detection of peripheral blood T lymphocyte subsets, the following antibodies were used: CD3-FITC/CD8-PE/CD4-APC (all from Becton-Dickinson). Cytofluorimetric analysis was performed with CytomicsTM FC500 (Beckman Coulter). CXP Cytometer and FlowJo Software (Tree Star Inc.) were used to analyze flow cytometry data. T lymphocyte subsets were identified as follows: CD3+ T cells, CD4+ T cells, and CD8+ T cells.
Li Y., Dong B., Wu W., Wang J., Jin H., Chen K., Huang K., Huang S, & Yao Y. (2022). Metagenomic Analyses Reveal Distinct Gut Microbiota Signature for Predicting the Neoadjuvant Chemotherapy Responsiveness in Breast Cancer Patients. Frontiers in Oncology, 12, 865121.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!