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Tubbiv

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The TubbIV is a versatile laboratory equipment designed for various applications. It functions as an incubator for cell culture and microbiology experiments, maintaining the optimal temperature, humidity, and atmospheric conditions required for the growth and maintenance of biological samples.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (6 mentions) Pdgfrα, by Cell Signaling Technology (3 mentions) Scgb1a1, by Santa Cruz Biotechnology (3 mentions) Type 1 collagen, by Abcam (2 mentions) Sm22α, by Abcam (2 mentions) Pdgfrβ, by Cell Signaling Technology (2 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (2 mentions) Proliferating cell nuclear antigen (pcna), by Biocare Medical (2 mentions) Mouse anti pcna, by Biocare Medical (2 mentions) Po4 histone h3, by Cell Signaling Technology (2 mentions) Mouse anti po4 histone h3, by Cell Signaling Technology (2 mentions) Anti sm22α, by Abcam (2 mentions) Bromodeoxyuridine (brdu), by Abcam (1 mentions) Sftpc, by Santa Cruz Biotechnology (1 mentions) Phospho histone h3, by Cell Signaling Technology (1 mentions) P smad2, by Merck Group (1 mentions) Hdac3, by Santa Cruz Biotechnology (1 mentions) C caspase 3, by Cell Signaling Technology (1 mentions)

3 protocols using tubbiv

1

Immunohistochemical Analysis of Mouse Lungs

Cited 1 time
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Mouse lungs were inflated and fixed in 2% paraformaldehyde, dehydrated in a series of increasing ethanol concentration washes, embedded in paraffin and sectioned. Antibodies used were anti-SM22α (goat anti-SM22α 1:200 Abcam), GFP (goat anti-GFP 1:100 Abcam, rabbit anti-GFP 1:100 Molecular Probe), Scgb1a1 (goat anti-Scgb1a1 1:20 Santa Cruz), SPC (rabbit-anti SPC 1:500 Chemicon), Pdgfrα (rabbit anti-Pdgfrα 1:50 Cell Signaling), Pdgfrβ (rabbit anti-Pdgfrβ 1:100 Cell Signaling), vimentin (rabbit anti-vimentin 1:100 Santa Crux), collagen type1 (rabbit anti-Col1 1:500 Abcam), Ki67 (rabbit anti-Ki67 1:50 Abcam), PCNA (mouse anti-PCNA 1:50 Biocare), PO4-Histone H3 (mouse anti-PO4-Histone H3 1:200 Cell Signaling), TubbIV (mouse anti-TubbIV 1:20 Biogenex), S100A4 (rabbit anti-S100A4 1:200 Abcam). LacZ staining of lungs was performed as previously described8 (link). The slide was imaged on a Zeiss LSM 710 confocal microscope and analyzed in ImageJ software.
Peng T., Frank D.B., Kadzik R.S., Morley M.P., Rathi K.S., Wang T., Zhou S., Cheng L., Lu M.M, & Morrisey E.E. (2015). Hedgehog actively maintains adult lung quiescence and regulates repair and regeneration. Nature, 526(7574), 578-582.
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2

Immunohistochemical Analysis of Mouse Lungs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse lungs were inflated and fixed in 2% paraformaldehyde, dehydrated in a series of increasing ethanol concentration washes, embedded in paraffin and sectioned. Antibodies used were anti-SM22α (goat anti-SM22α 1:200 Abcam), GFP (goat anti-GFP 1:100 Abcam, rabbit anti-GFP 1:100 Molecular Probe), Scgb1a1 (goat anti-Scgb1a1 1:20 Santa Cruz), SPC (rabbit-anti SPC 1:500 Chemicon), Pdgfrα (rabbit anti-Pdgfrα 1:50 Cell Signaling), Pdgfrβ (rabbit anti-Pdgfrβ 1:100 Cell Signaling), vimentin (rabbit anti-vimentin 1:100 Santa Crux), collagen type1 (rabbit anti-Col1 1:500 Abcam), Ki67 (rabbit anti-Ki67 1:50 Abcam), PCNA (mouse anti-PCNA 1:50 Biocare), PO4-Histone H3 (mouse anti-PO4-Histone H3 1:200 Cell Signaling), TubbIV (mouse anti-TubbIV 1:20 Biogenex), S100A4 (rabbit anti-S100A4 1:200 Abcam). LacZ staining of lungs was performed as previously described8 (link). The slide was imaged on a Zeiss LSM 710 confocal microscope and analyzed in ImageJ software.
Peng T., Frank D.B., Kadzik R.S., Morley M.P., Rathi K.S., Wang T., Zhou S., Cheng L., Lu M.M, & Morrisey E.E. (2015). Hedgehog actively maintains adult lung quiescence and regulates repair and regeneration. Nature, 526(7574), 578-582.
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3

Immunohistochemical Analysis of Lung Development

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were fixed in 4% paraformaldehyde overnight and subject to paraffin sectioning. Immunohistochemistry was performed by using the following antibodies and concentrations: Hdac3 (Santa Cruz Biotechnology, 1:10), Nkx2.1 (Santa Cruz, 1:50), p-Smad2 (Millipore, 1:1000), Sox2 (Seven Hills, 1:500), Sox9 (Santa Cruz, 1:100), Sftpc (Santa Cruz, 1:50), Sftpb (Chemicon, 1:100), Aqp5 (Abcam, 1:100), Pdpn (Hybridoma Bank, 1:50), Scgb1a1 (Santa Cruz, 1:20), TubbIV (BioGenex, 1:20), Pdgfrα (Cell Signaling, 1:25), Pecam (Pharmingen, 1:500), Col4a3 (Santa Cruz, 1:50), RAGE (R&D Systems, 1:50) c-Caspase 3 (Cell Signaling, 1:50), BrdU (Abcam, 1:100), and phospho-histone H3 (Cell Signaling, 1:200). Whole-mount staining was performed as previously described (Metzger et al., 2008 (link)).
Wang Y., Frank D.B., Morley M.P., Zhou S., Wang X., Lu M.M., Lazar M.A, & Morrisey E.E. (2016). HDAC3-Dependent Epigenetic Pathway Controls Lung Alveolar Epithelial Cell Remodeling and Spreading via miR-17-92 and TGF-β Signaling Regulation. Developmental cell, 36(3), 303-315.
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