The partial sequences of BCYRN1 and the 3′-untranslated region (3′-UTR) of KRAS containing the putative binding sites of miR-204-3p were synthesized and inserted into a luciferase reporter vector plasmid (GenePharma, Shanghai, China). According to the instructions, firefly luciferase reporter plasmids and equal amounts of miR-204-3p mimics or NC mimics were transfected into cells. Then, the relative luciferase activities were detected by the Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA) on a Luminometer 20/20n (Turmer Biosystems, Sunnyvale, CA, USA) after transfection for 48 h. Renilla luciferase activity was employed as an internal control for cellular density and transfection efficiency.
Luciferase reporter vector plasmid
Manufactured by GenePharma
Sourced in China
Luciferase reporter vector plasmid is a laboratory tool used for gene expression analysis. It contains a luciferase gene that can be used as a reporter to measure the activity of a promoter or regulatory sequence of interest. The luciferase enzyme catalyzes a bioluminescent reaction, providing a quantifiable readout of gene expression levels.
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Lab products found in correlation
4 protocols using luciferase reporter vector plasmid
1
Luciferase Assay for BCYRN1 and KRAS
2
Luciferase Assay of miR-204-3p Binding
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The partial sequences of BCYRN1 and 3'-untranslated region (3'-UTR) of KRAS containing the putative binding sites of miR-204-3p were synthesized and inserted into a luciferase reporter vector plasmid (Genepharma, China). According to the instruction, re y luciferase reporter plasmid, equal amounts of miR-204-3p mimics or NC mimics were transfected into cells, respectively. Then, the relative luciferase activities were detected by the Dual-Luciferase Reporter Assay System (Promega, USA) on Luminometer 20/20n (Turmer Biosystems, USA) after transfection for 48 h. Renilla luciferase activity was employed as an internal control for cellular density and transfection e ciency.
3
Luciferase Assay for miR-204-3p Targets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The partial sequences of BCYRN1 and the 3'-untranslated region (3'-UTR) of KRAS containing the putative binding sites of miR-204-3p were synthesized and inserted into a luciferase reporter vector plasmid (GenePharma, Shanghai, China). According to the instructions, re y luciferase reporter plasmids and equal amounts of miR-204-3p mimics or NC mimics were transfected into cells. Then, the relative luciferase activities were detected by the Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA) on a Luminometer 20/20n (Turmer Biosystems, Sunnyvale, CA, USA) after transfection for 48 h. Renilla luciferase activity was employed as an internal control for cellular density and transfection e ciency.
4
Validating miR-204-3p Binding to BCYRN1 and KRAS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The partial sequences of BCYRN1 and 3'-untranslated region (3'-UTR) of KRAS containing the putative binding sites of miR-204-3p were synthesized and inserted into a luciferase reporter vector plasmid (Genepharma, China). According to the instruction, re y luciferase reporter plasmid, equal amounts of miR-204-3p mimics or negative control mimics (NC) were transfected into cells, respectively. Then, the relative luciferase activities were detected by the Dual-Luciferase Reporter Assay System (Promega, USA) on Luminometer 20/20n (Turmer Biosystems, USA) after transfection for 48 h.
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