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Pb0375

Manufactured by Boster Bio
Sourced in China

The PB0375 is a laboratory centrifuge designed for general-purpose sample separation and processing. It is capable of high-speed rotation to separate particles, cells, or other components from liquid samples. The centrifuge provides consistent and reliable performance for a wide range of applications in clinical, research, and industrial settings.

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3 protocols using pb0375

1

Immunofluorescence Staining of Frozen Kidney Sections

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Immunofluorescence staining was performed with routine protocol [72 (link)]. Frozen kidney sections (3 μm) were fixed with 4% paraformaldehyde. All primary antibodies used were as follows: anti-TOMM20 (ab186735; Abcam), anti-Sirtuin 6 (PB0375; Boster), anti-PGC-1α (ab54481; Abcam). Then the slides were stained with Cy3-or Cy2-conjugated secondary antibodies (Jackson Immuno-Research Laboratories, West Grove, PA, United States). Nuclei were treated with DAPI (Sigma-Aldrich) under the manufacturer’s specifications.
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2

Immunohistochemical Staining of Kidney Sections

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Paraffin kidney sections (3 μm) were stained with periodic acid–Schiff (PAS) reagent by standard protocol. Immunohistochemical staining was performed with routine protocol [37 (link)]. Antibodies used were as follows: anti-caspase-3 (SC-65497; Santa Cruz Biotechnology), anti-KIM-1 (BA3537; Boster), anti-Sirtuin 6 (PB0375; Boster).
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3

Comprehensive Protein Expression Analysis

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Protein expression was analyzed by Western blot analysis. The primary antibodies were as follows: anti-AR (BA34204; Boster), anti-Bax (SC-7480; Santa Cruz Biotechnology), anti-caspase-3 (SC-65497; Santa Cruz Biotechnology), anti-Flag-tag (M185-3S; MBL), anti-FAS-L (SC-19681; Santa Cruz Biotechnology), anti-GAPDH (RM2001; Ray Antibody Biotech), anti-KIM-1 (BA3537; Boster), anti-PGC-1α (ab54481; Abcam), anti-Sirtuin 1 (PB0173; Boster), anti-Sirtuin 2 (PB9160; Boster), anti-Sirtuin 3 (PB0175; Boster), anti-Sirtuin 4 (A15800; ABclonal), anti-Sirtuin 5 (ab108968; Abcam), anti-Sirtuin 6 (PB0375; Boster), anti-Sirtuin 7 (PB0376; Boster), anti-TOMM20 (ab186735; Abcam), anti-α-tubulin (RM2007; Ray Antibody Biotech, Beijing, China), anti-β-actin (RM2001; Beijing Ray Antibody Biotech).
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