Tripterine (purity >99.9%) was obtained from Sinopharm Group Co, Ltd (Shanghai, China). Coix seed oil (purity >85%) was extracted by supercritical fluid extraction.40 Cremophor® RH 40 was provided by BASF Co, Ltd (Ludwigshafen, Germany). PEG 400 was purchased from Sigma-Aldrich Co (St Louis, MO, USA). Tf was purchased from Tufeng Biology Co, Ltd (Shanghai, China). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were offered by TCI Development Co, Ltd (Shanghai, China). HeLa cells were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, China). MTT was sourced from Amresco, Inc (Solon, OH, USA). Amiloride, sucrose, genistein, and ammonium chloride were purchased from Sinopharm Group Co, Ltd. Experimental water was produced by Milli-Q water purification system (Merck Millipore, Billerica, MA, USA). All other chemicals were of analytical grade unless stated otherwise.
Genistein
Manufactured by Sinopharm
Sourced in United States, China
Genistein is a laboratory reagent used in biochemical research and analysis. It is a naturally occurring isoflavone compound found in various plants. Genistein functions as a tyrosine kinase inhibitor, which is a key role in its use for in-vitro studies.
Automatically generated - may contain errors
Lab products found in correlation
Amiloride, by Sinopharm (2 mentions)
Cremophor rh40, by BASF (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Avantor (1 mentions)
Tripterine, by Sinopharm (1 mentions)
Peg400, by Merck Group (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Sucrose, by Sinopharm (1 mentions)
Ammonium chloride, by Sinopharm (1 mentions)
Ammonia chloride, by Sinopharm (1 mentions)
Elix 5 milli q water purification system, by Merck Group (1 mentions)
Fluorescein isothiocyanate (fitc), by Beyotime (1 mentions)
Mtt cell proliferation and cytotoxicity detection kit, by Keygen Biotech (1 mentions)
Etoposide, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Keygen Biotech (1 mentions)
1640 medium, by Keygen Biotech (1 mentions)
Peg400, by Sinopharm (1 mentions)
Fetal bovine serum (fbs), by Keygen Biotech (1 mentions)
2 protocols using genistein
1
Tripterine-Based Nanomedicine Development
2
Coix Seed Oil Extraction and Characterization
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Coix seed oil was extracted from Coix Lacryma-jobi by our group (Qu et al., 2014 ), the purity was determined as 87.6% using glycerin trilaurate as a reference. G-Rh2 (purity >98%) was purchased from Nanjing Zelang BioTECH Co. (Jiangsu, China). Etoposide (purity >99.9%) was bought from Sigma-Aldrich Co., Ltd. (Poole, UK). Cremophor® RH40 was provided by BASF Co., Ltd. (Ludwigshafen, Germany). PEG400, amiloride, genistein, chlorpromazine, and ammonia chloride were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Roswell park memorial institute (RPMI) 1640 medium, fetal bovine serum (FBS), penicillin–streptomycin solution, MTT cell proliferation and cytotoxicity detection kit were bought from KeyGEN BioTECH Co., Ltd. (Jiangsu, China). The fluorescence probes including DiI and fluorescein isothiocyanate (FITC) were purchased from Beyotime Biotech Co., Ltd. (Shanghai, China). The water used in this study was produced by Elix®5 Milli Q-water purification system (Millipore, Billerica, MA). The other chemical reagents were of analytical grade, unless otherwise statement.
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