The organic acids were analyzed by HPLC (Jasco) with UV-Visible detection (Jasco 870-UV-visible). The chromatographic separation was performed on an Aminex HPX-87H column (300 × 4.6 mm, 8 μm). A 5 M H2SO4 solution was filtered and degassed to use as eluent. The flow rate was set to 0.6 mL/min at 60 °C, and 20 μL were injected. Detection was carried out at 210 nm. ChromPass software (Jasco Deutschland) was used to analyze the chromatographic data. For each organic acid, individual stock solutions were prepared by dissolving the compounds in distillate H2O, and seven-point calibration curves were prepared at concentrations of 0.1–2 mg/mL for acetic acid (AA), 1–20 mg/mL for lactic acid (LA), and 0.05–0.7 mg/mL for citric acid. Peak areas were used for quantification.
870 uv visible
Manufactured by Jasco
Sourced in Spain
The 870-UV-visible is a laboratory instrument used for spectrophotometric analysis. It measures the absorbance or transmittance of light through a sample across a range of ultraviolet and visible wavelengths.
Automatically generated - may contain errors
2 protocols using 870 uv visible
1
HPLC Analysis of Organic Acids
2
Comprehensive Chemical Analysis of Food Samples
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The pH was measured according to the methodology proposed by the Association of
Official Analytical Chemists (AOAC) International, at room temperature, using a
digital pH meter (Micronal B474 model, São Paulo, Brazil) (AOAC, 2000 ).
Moisture, dry matter, fat, and ash content were determined according to the
methodology proposed by AOAC (2000) . Total
nitrogen content was determined using the Kjeldahl method, and crude protein
content was calculated using the conversion factor 6.25. The concentration of
carbohydrates was determined as 100 - (% moisture + % protein + % fat + % ash
content), and energy value was calculated using the Atwater method (Wisker and Feldheim, 1990 (link)).
The concentrations of alcohols (ethanol, glycerol, and methanol), organic acids
(oxalic, citric, tartaric, malic, succinic, propionic, butyric, acetic, and
lactic), and carbohydrates (glucose, sucrose, fructose, maltose, and raffinose)
were determined by HPLC accordingDuarte
et al. (2010) using a Jasco chromatograph equipped
with a refractive index (RI) detector (Jasco 830-RI, Madrid, Spain) and UV-Visible
detector (Jasco 870-UV-visible). A Chrompack column (300 mm x 6.5 mm) at 60 °C,
using 5 mM sulphuric acid as the eluent, at a flow rate of 0.5 mL min-1and a sample volume of 20 μL was used.
Official Analytical Chemists (AOAC) International, at room temperature, using a
digital pH meter (Micronal B474 model, São Paulo, Brazil) (AOAC, 2000 ).
Moisture, dry matter, fat, and ash content were determined according to the
methodology proposed by AOAC (2000) . Total
nitrogen content was determined using the Kjeldahl method, and crude protein
content was calculated using the conversion factor 6.25. The concentration of
carbohydrates was determined as 100 - (% moisture + % protein + % fat + % ash
content), and energy value was calculated using the Atwater method (Wisker and Feldheim, 1990 (link)).
The concentrations of alcohols (ethanol, glycerol, and methanol), organic acids
(oxalic, citric, tartaric, malic, succinic, propionic, butyric, acetic, and
lactic), and carbohydrates (glucose, sucrose, fructose, maltose, and raffinose)
were determined by HPLC according
et al. (2010)
with a refractive index (RI) detector (Jasco 830-RI, Madrid, Spain) and UV-Visible
detector (Jasco 870-UV-visible). A Chrompack column (300 mm x 6.5 mm) at 60 °C,
using 5 mM sulphuric acid as the eluent, at a flow rate of 0.5 mL min-1and a sample volume of 20 μL was used.
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