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2 protocols using luvitec mw 64 kda

1

Microparticle Synthesis for Biomedical Applications

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Poly(ethylene glycol) diacrylate (Mn 700) (PEG700DA), 2-aminoethyl methacrylate hydrochloride (AEM), and diphenyl (2,4,6-trimethylbenzoyl)-phosphine oxide (TPO) were purchased from Sigma-Aldrich. Thermo Scientific Dylight 488, PTFE syringe filters (13 mm membrane, 0.22 μm pore size), sterile water, and methanol were obtained from Fisher Scientific. Conventional filters (2 μm) were purchased from Agilent, polyvinyl alcohol (Mw 2000) (PVOH) was purchased from Acros Organics, and Luvitec (MW 64 kDa) was purchased from BASF. PRINT molds (80 nm x 320 nm, 80 nm x 5000 nm, and 200 nm x 200 nm) were obtained from Liquidia Technologies. Tetraethylene glycol monoacrylate (HP4A) was synthesized in-house as previously described [39 ].
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2

Fabrication of Biodegradable Nanoparticles

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Poly(ethylene glycol) diacrylate (Mn 700) (PEG700DA), 2-aminoethyl methacrylate hydrochloride (AEM), and diphenyl (2,4,6-trimethylbenzoyl)-phosphine oxide (TPO) were purchased from Sigma-Aldrich. Thermo Scientific Dylight 488, PTFE syringe filters (13 mm membrane, 0.22 μm pore size), sterile water, and methanol were obtained from Fisher Scientific. Conventional filters (2 μm) were purchased from Agilent, polyvinyl alcohol (Mw 2000) (PVOH) was purchased from Acros Organics, and Luvitec (MW 64 kDa) was purchased from BASF. PRINT molds (80 nm x 320 nm, 80 nm x 5000 nm, and 200 nm x 200 nm) were obtained from Liquidia Technologies. Tetraethylene glycol monoacrylate (HP4A) was synthesized in-house as previously described [30 ].
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