Cells re-suspended in 100 µl FACS Buffer were incubated with Fc blocker anti-CD16/32 antibody (101319, Biolegend) for 10 min on ice, followed by staining with fluorochrome-conjugated antibody on ice for 30 min. The antibodies used in this study include anti-mouse CD140a (Pdgfra) APC (17-1401-81, eBioscience, 1:100), goat-anti-Lepr-biotin (AF497, R&D, 1:100), anti-Ter119-PE-cy7 (25-5921-81, eBioscience, 1:1000), anti-CD31-PE-cy7 (25-0311-81, eBioscience, 1:1000), and anti-CD31-PE-cy7 (25-0451-81, eBioscience, 1:1000). Samples stained with biotin-conjugated antibodies were washed with FACS Buffer and then incubated with streptavidin-brilliant violet 421 (Biolegend, 1:500) for 20 min. Flow cytometric analyses were carried out with an Lsr Fortessa flow cytometer equipped with the FACS Diva 6.1 software (BD Biosciences). Data were analyzed with FlowJo version 9.
Goat anti lepr biotin af497
Manufactured by R&D Systems
Goat-anti-Lepr-biotin (AF497) is a biotinylated antibody raised against the Leptin Receptor (Lepr) protein in goats. This product can be used for detection and quantification of Lepr in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse cd140a pdgfra apc, by Thermo Fisher Scientific (1 mentions)
Streptavidin brilliant violet 421, by BioLegend (1 mentions)
Anti cd31 pe cy7, by Thermo Fisher Scientific (1 mentions)
Facsdiva 6, by BD (1 mentions)
Lsrfortessa flow cytometer, by BD (1 mentions)
Streptavidin bv421, by BioLegend (1 mentions)
Streptavidin apc efluor 780, by Thermo Fisher Scientific (1 mentions)
Facsaria flow cytometer, by BD (1 mentions)
Ghost dye red 780, by Cytek Biosciences (1 mentions)
Anti sca 1 d7, by Thermo Fisher Scientific (1 mentions)
Facsdiva 8, by BD (1 mentions)
2 protocols using goat anti lepr biotin af497
1
Flow Cytometry Immunophenotyping of Cells
2
Flow Cytometry Staining of Biomarkers
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Samples were stained with combinations of the following antibodies for flow cytometry: Goat-anti-LepR-biotin (AF497, R&D Systems), anti-CD45 (30F-11, Tonbo Biosciences), anti-CD144 (clone BV13, eBiosciences), anti-TER119 (Tonbo Biosciences), anti-Sca1(D7, eBiosciences), anti-CD51-biotin (RMV-7, BioLegend), Streptavidin BV421 (BioLegend), and/or Streptavidin APCeFluor 780 (eBiosciences). All staining was performed for 1.5 h on ice. Dead cells were identified and gated out of sorts by including 4 0 ,6-diamidino-2-phenylindole (DAPI) (1 mg/mL) in the buffer in which cells were resuspended for flow cytometry or by staining cells with Ghost Dye Red 780 (1:100, Tonbo Biosciences) before resuspending cells for flow cytometric analysis. Samples were analyzed or sorted using FACSAria flow cytometers and FACSDiva 8.0 (BD) or FlowJo v10.6.1 (Tree Star) software.
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