Mission sirna universal control 1

Manufactured by Merck Group

Sourced in United States

MISSION siRNA universal control#1 is a laboratory product offered by Merck Group. It is designed to serve as a control in siRNA-based experiments. The product provides a non-targeting siRNA sequence that can be used to assess non-specific effects of siRNA transfection.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Fugene hd, by Promega (1 mentions)

Spelling variants of this product

SiRNAs (112 citations) Control siRNA (99 citations) MISSION siRNA (87 citations) MISSION control siRNA (2 citations)

2 protocols using mission sirna universal control 1

Kcne4 siRNA Transfection in LECs

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A total of 1.5 × 10⁵ LECs were transfected with 25 pmol non-targeting siRNA (MISSION siRNA universal control#1, MERCK, Darmstadt, Germany) or siRNA against Kcne4 (Table S3) using Lipofectamine RNAiMAX (Thermo Fisher Science). Mouse Kcne4 cDNA was cloned by PCR and inserted into pcDNA3.1 plasmid. pcDNA3.1-KCNE4 plasmid was transfected into LECs using Fugene HD (Promega, Madison, WI). Transfection was performed in accordance with the manufacturer’s instructions.

Mano R., Tanaka T., Hashiguchi S., Takahashi H., Sakata N., Kondo S, & Kodama S. (2022). Induction of potassium channel regulator KCNE4 in a submandibular lymph node metastasis model. Scientific Reports, 12, 13208.

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Suppression of Integrins and DUSP6 by siRNA

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Expression of integrins and DUSP6 was suppressed by siRNA. siRNAs were designed by an siRNA design site, siDirect ver.2 (http://sidirect2.rnai.jp/). Sense and complementary strand RNAs having a two-mer overhang were synthesized and annealed. The siRNA sequences for each mRNA target are listed in Table S2. LECs (1.5 × 10⁵) were transfected with 25 pmol of target siRNA or non-target siRNA (MISSION siRNA universal control#1, Merck) using Lipofectamine RNAiMAX (Thermo Fisher, Waltham, MA, USA) according to the manufacturer’s instructions.

Hashiguchi S., Tanaka T., Mano R., Kondo S, & Kodama S. (2022). CCN2-induced lymphangiogenesis is mediated by the integrin αvβ5–ERK pathway and regulated by DUSP6. Scientific Reports, 12, 926.

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