Novex tris glycine 4 20 precast gels

Manufactured by Thermo Fisher Scientific

Novex Tris-Glycine 4–20% precast gels are polyacrylamide gels used for protein separation and analysis in electrophoresis procedures. They have a gradient concentration of 4-20% acrylamide, which allows for the separation of a wide range of protein sizes. These gels are pre-cast, ready-to-use, and designed for use with Tris-Glycine buffer systems.

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Lab products found in correlation

Hrp conjugated secondary antibody, by Bio-Rad (2 mentions)

Close spelling variants of this product

Novex precast 4–20% tris-glycine polyacrylamide gels Novex 4–20% Tris-Glycine gels Novex Tris-Glycine 4-20% gels Novex Tris–glycine precast gels 4–20% Tris–Glycine precast gel Precast tris-glycine gels Novex Tris-Glycine gels Novex precast gels Tris-Glycine gels Precast gels

2 protocols using novex tris glycine 4 20 precast gels

Western Blot Protein Analysis

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Samples were boiled in 2× SDS-PAGE loading buffer (78.0 mM Tris [pH 6.8], 4% SDS, 20% glycerol, 100 mM DTT, 0.2% bromophenol blue) and loaded on SDS-PAGE gels (fixed % between 10 and 15% acrylamide [37.5:1], Bio-Rad, or Novex Tris-Glycine 4–20% precast gels [Invitrogen]). Proteins were transferred onto PVDF (Immobilon), which were blocked in TBS-T containing 5% nonfat milk or BSA before primary antibodies were added overnight at 4 °C. After three TBS-T washes, membranes were incubated with HRP-conjugated secondary antibodies (Bio-Rad, 1:10,000) and developed with ECL reagents (Pierce). Antibodies used are in supplemental Table S2.

Siddaway R., Milos S., Coyaud É., Yun H.Y., Morcos S.M., Pajovic S., Campos E.I., Raught B, & Hawkins C. (2022). The in vivo Interaction Landscape of Histones H3.1 and H3.3. Molecular & Cellular Proteomics : MCP, 21(10), 100411.

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Western Blot Protein Analysis Protocol

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Whole lysates were prepared in 2 × SDS-PAGE buffer (78.0 mM Tris [pH 6.8], 4% SDS, 20% glycerol, 100 mM DTT, 0.2% bromophenol blue) and separated on Novex Tris-Glycine 4–20% precast gels (Invitrogen). After transfer to PVDF membranes (Immobilon) that were then blocked in TBS-T containing 5% nonfat milk or BSA, primary antibodies were added overnight at 4 °C. After 3 TBS-T washes, membranes were incubated with HRP-conjugated secondary antibodies (BioRad, 1:10,000) and developed with ECL reagents (Pierce). Quantification was done with ImageJ.

Siddaway R., Canty L., Pajovic S., Milos S., Coyaud E., Sbergio S.G., Vadivel Anguraj A.K., Lubanszky E., Yun H.Y., Portante A., Carette S., Zhang C., Moran M.F., Raught B., Campos E.I, & Hawkins C. (2022). Oncohistone interactome profiling uncovers contrasting oncogenic mechanisms and identifies potential therapeutic targets in high grade glioma. Acta Neuropathologica, 144(5), 1027-1048.

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