The following primary antibodies were used on sections at 1:100 dilution unless otherwise indicated: Claudin5 (Santa Cruz, sc28670), Connexin40 (Santa Cruz, sc20466), E-cadherin (BD Transduction, 610182), Endomucin (Santa Cruz, sc65495), GFP (Aves Labs, GFP-1020, 1:500), Nrp1 (R&D Sytems, AF566), Nrp2 (Cell Signaling, 3366s), PECAM-1 (BD Transduction, 553370), Podocalyxin (R&D Systems, AF1556), smooth muscle alpha (Abcam, ab14106), VE-cadherin (Santa Cruz, sc6458), VEGF (Abcam, ab14708), ZO1 (Invitrogen, 339100).
The following primary antibodies were used in whole mount staining at indicated dilutions: Connexin40 (Santa Cruz, sc20466, 1:100), E-cadherin (BD Transduction, 610182, 1:100), Endomucin (Santa Cruz, sc65495, 1:400), GFP (Aves Labs, GFP-1020, 1:500), PECAM-1 (BD Transduction, 553370, 1:400).
Secondary antibodies were used at 1:500 dilution: Alexa goat α mouse-488, Alexa goat α mouse-555, Alexa donkey α mouse-555, Alexa donkey α rabbit-555, Alexa chicken α rat-488, Alexa goat α rat-555, Alexa donkey α goat-555, Alexa donkey α goat-488, Alexa donkey α chicken-488.
Azizoglu D.B., Chong D.C., Villasenor A., Magenheim J., Barry D.M., Lee S., Marty-Santos L., Fu S., Dor Y, & Cleaver O. (2016). Vascular development in the vertebrate pancreas. Developmental biology, 420(1), 67-78.
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