At the heading stage, 10 randomly selected WT and spl36 leaves of obvious phenotype and similar growth were stained with trypan blue and DAB solution according to the method of Dietrich et al. (1994) (link) and Thordal-Christensen et al. (1997) . Meanwhile, the first, second, and third leaves of the WT and spl36 mutant were collected and used to measure ROS and proline content, as well as catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) activity as previously described. The Superoxide Anion Assay Kit (A052), Proline Content Assay Kit (A107), SOD Activity Assay Kit (A001-1), CAT Assay Kit (A007-1), and POD Activity Assay Kit (A084-3) were provided by the Nanjing Jiancheng Technology Company (Nanjing, China).
Pod activity assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The POD Activity Assay Kit is a laboratory equipment product designed to measure the activity of peroxidase (POD) enzymes. It provides a quantitative colorimetric method for determining POD activity in various samples.
Automatically generated - may contain errors
Lab products found in correlation
Sod activity assay kit, by Nanjing Jiancheng (2 mentions)
Superoxide anion assay kit, by Nanjing Jiancheng (1 mentions)
Proline content assay kit, by Nanjing Jiancheng (1 mentions)
Cat assay kit, by Nanjing Jiancheng (1 mentions)
Ldh activity assay kit, by Nanjing Jiancheng (1 mentions)
Cat activity assay kit, by Nanjing Jiancheng (1 mentions)
Mda assay kit, by Nanjing Jiancheng (1 mentions)
2 protocols using pod activity assay kit
1
Oxidative Stress Response in Arabidopsis spl36 Mutant
2
Antioxidant Enzymes and Oxidative Stress
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The activity of lactate dehydrogenase (LDH), TrxR, anti-superoxide anion radical (AntiO 2 -), superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and the contents of malondialdehyde (MDA) in the culture medium and plasma were determined according to LDH activity assay kit (Colorimetric method), TrxR activity assay kit (Colorimetric method), AntiO 2 -activity assay kit (enzyme-linked immunosorbent assay method), SOD activity assay kit (WST-1 cell proliferation reagent method), CAT activity assay kit (Colorimetric method), POD activity assay kit (Colorimetric method) and MDA assay kit (thiobarbituric acid method) (Nanjing Jiancheng Bioengineering company, Jiangsu, China), respectively.
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