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Si circtp63

Manufactured by GenePharma
Sourced in China

Si-circTP63 is a laboratory equipment designed for the detection and analysis of circular RNA (circRNA) containing the TP63 gene. It is a specialized tool used in molecular biology and genomics research. The core function of Si-circTP63 is to facilitate the identification and quantification of TP63-containing circRNAs, which can provide insights into gene expression and regulation.

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3 protocols using si circtp63

1

Transfection of circTP63 and miR-217

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Cell transfection was performed with Lipofectamine 2000 or 3000 Reagent (Invitrogen, CA, USA) according to the manufacturer’s protocol. The two siRNA against circTP63 and the si-negative control (si-NC) oligos were purchased from Gene Pharma (Shanghai, China). The sequences for si-circTP63-1, si-circTP63-2 or si-NC were as follows: si-circTP63-1,5′-GCCAACAGUGAGGGGCCGU-3′; si-circTP63-2,5′-CAACAGUGAGGGGCCGUGAGA-3′; si-NC, 5′-UUCUCCGAACGUGUCACGU-3′. MiR-217 mimic, miR-217 inhibitor and miR-NC were obtained from Gene Pharma (Shanghai, China).
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2

Transfection of circTP63 siRNA and miR-217

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Cell transfection was performed with Lipofectamine 2000 or 3000 Reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. The siRNA sequences for transfection were purchased from GenePharma (Shanghai, China).The sequences for circTP63 was as follows:si-circTP63-1, 5'-GCCAACAGUGAGGGGCCGU-3';si-circTP63-2,5'-CAACAGUGAGGGGCCGUGAGA-3';siRNAcontrol(si-NC)5'-UUCUCCGAACGUGUCACGU-3'.MiR-217mimic, miR-217 inhibitor and miR-NC were obtained from Gene Pharma (Shanghai, China).
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3

Transfection of circTP63 siRNA and miR-217

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell transfection was performed with Lipofectamine 2000 or 3000 Reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. The siRNA sequences for transfection were purchased from GenePharma (Shanghai, China).The sequences for circTP63 was as follows:si-circTP63-1, 5'-GCCAACAGUGAGGGGCCGU-3';si-circTP63-2,5'-CAACAGUGAGGGGCCGUGAGA-3';siRNAcontrol(si-NC)5'-UUCUCCGAACGUGUCACGU-3'.MiR-217mimic, miR-217 inhibitor and miR-NC were obtained from Gene Pharma (Shanghai, China).
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