Primary antibodies and titers used in this study for immunostaining are as follows: Rabbit anti-Cleaved Caspase-3 (Asp175) antibody (Cell Signaling, 1:200), mouse anti-Elav antibody (Developmental Studies Hybridoma Bank, DSHB, 1:50), mouse anti-Repo (Developmental Studies Hybridoma Bank, DSHB, 1:50), rabbit anti-GFP (GeneTex, 1:200), mouse anti-NimC1 (a gift from Dr István Andó, 1:30)24 (link), rabbit anti-Eiger (a gift from Dr Chun-Hong Chen, National Health Research institute, NHRI, 1:200), rabbit anti-FasII (a gift from Dr Vivian Budnik, 1:5000)42 (link) and rabbit anti-pFAK (Cell Signaling, 1:200). For western blotting, mouse anti-Aβ42 (6E10) (Covance, 1:5000), rabbit anti-phospho JNK (pTPpY) (Promega, 1:2000), rabbit anti-JNK (Santa Cruz, 1:5000), and rabbit anti-GFP (GeneTex, 1:5000) were used.
Mouse anti aβ42 6e10
Manufactured by Fortrea
Sourced in Germany
Mouse anti-Aβ42 (6E10) is a monoclonal antibody that specifically recognizes the amyloid-beta 42 (Aβ42) peptide. It is a widely used tool in research applications for the detection and analysis of Aβ42.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti cleaved caspase 3 asp175 antibody, by Cell Signaling Technology (1 mentions)
Mouse anti elav antibody, by Developmental Studies Hybridoma Bank (1 mentions)
Rabbit anti gfp, by GeneTex (1 mentions)
Rabbit anti p fak, by Cell Signaling Technology (1 mentions)
Rabbit anti jnk, by Santa Cruz Biotechnology (1 mentions)
Mouse anti repo, by Developmental Studies Hybridoma Bank (1 mentions)
Mouse anti β tubulin, by Abcam (1 mentions)
Sp5 confocal microscope, by Leica (1 mentions)
2 protocols using mouse anti aβ42 6e10
1
Immunostaining and Western Blot Protocols
2
Immunofluorescence Analysis of Neuronal Markers
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Adults were dissected and fixed with 4% formaldehyde in phosphate-buffered saline (PBS-1X) for 20 min, washed three times with PBS-1 × 0.1% Triton X-100, and mounted in Vectashield medium with DAPI (4′,6-diamidino-2-phenylindole), or incubated with primary and secondary antibodies. The following antibodies and dilutions were used: mouse anti-Bruchpilot (nc82) 1:20 (DSHB); rabbit anti-HRP 1:200 (DSHB); mouse anti-Aβ42 (6E10) 1:1500 (Covance); mouse anti-phospho-Ser 1:200 (Abcam); rabbit anti-phospho-Tyr 1:200 (Abcam); mouse anti-phosphoSer8-Aβ42 1:1000, and rat anti-phosphoSer26-Aβ42 1:1000 (W. Jochen, Universitätklinikum Bonn, Germany); mouse anti-β-tubulin 1:10,000 (Abcam). As secondary antibodies, we used anti-mouse Alexa 488 and anti-rabbit Alexa 568. Preparations were imaged in a Leica SP5 confocal microscope and images were processed by ImageJ. Fluorescence quantification was performed with Imaris (Bitplane) software.
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