Arachidonoyl-CoA, 5-sulfosalicilic acid (SSA), ammonium formate solution, glacial acetic acid, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). Optima LC-MS grade methanol, acetonitrile (ACN), 2-propanol (IPA) and water were purchased from Fisher Scientific (Pittsburgh, PA). 2-(2-pyridyl) ethyl functionalized silica gel 100 mg/1 mL tubes were obtained from Supelco Analytical (Bellefonte, PA). F-12K media, DMEM, medium 200, low serum growth supplement (LSGS) kit, streptomycin, and penicillin were purchased from Invitrogen (Carlsbad, CA). Charcoal stripped fetal bovine serum (csFBS) was from Gemini Bioproducts (West Sacramento, CA). [13C315N1] calcium pantothenate was purchased from Isosciences (King of Prussia, PA). Peroxide free arachidonic acid (AA) was purchased from Cayman Chemical (Ann Arbor, MI). Human colorectal adenocarcinoma (LoVo) cells and human colon adenocarcinoma (HCA7) cells were obtained from ATCC (Manassas, VA). Livers from C57BL/6 mice were taken under University of Pennsylvania IACUC protocol # 803875.
Charcoal stripped fetal bovine serum
Manufactured by Gemini Bio
Charcoal stripped fetal bovine serum (csFBS) is a specialized laboratory reagent. It is produced by processing fetal bovine serum through a charcoal adsorption process, which removes various lipophilic components, including hormones, lipids, and other small molecules.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
2 propanol, by Thermo Fisher Scientific (1 mentions)
Low serum growth supplement kit, by Thermo Fisher Scientific (1 mentions)
Medium 200, by Thermo Fisher Scientific (1 mentions)
Arachidonoyl coa, by Merck Group (1 mentions)
Peroxide free arachidonic acid, by Cayman Chemical (1 mentions)
Optima lc ms grade methanol, by Thermo Fisher Scientific (1 mentions)
F 12k media, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
2 2 pyridyl ethyl functionalized silica gel 100 mg 1 ml tubes, by Merck Group (1 mentions)
Ici 182 780, by Bio-Techne (1 mentions)
T rex system, by Thermo Fisher Scientific (1 mentions)
Doxycycline dox, by Merck Group (1 mentions)
Z 4 hydroxytamoxifen, by Merck Group (1 mentions)
Way 200070, by Bio-Techne (1 mentions)
Liquiritigenin, by Bio-Techne (1 mentions)
2 protocols using charcoal stripped fetal bovine serum
1
Lipidomics Analysis of Colon Cancer Cells
2
Regulation of Breast Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Parental and ERβ1-expressing MCF7 cells [12 (link)] and doxycycline-inducible Hs578T-ERβ1 cells [8 (link)] were cultured as previously described. Doxycycline-inducible ERβ1-expressing MDA-MB-231 cell lines were established using the T-REx™ System (Invitrogen) as previously described [9 (link)] and were maintained in DMEM/F12 medium supplemented with 10% FBS, 1% AA, 5 mg/L blasticidin S and 500 mg/L zeocin. Charcoal-stripped fetal bovine serum (CS-FBS) was purchased from Gemini Bio-Products (West Sacramento, CA). 17β-estradiol (E2), (Z)-tamoxifen, (Z)-4-hydroxy-tamoxifen and doxycycline (Dox) were purchased from Sigma-Aldrich (St. Louis, MO). (Z)-endoxifen was synthesized by the National Cancer Institute (Bethesda, MD). The ERβ-specific agonists; DPN, WAY200070, FERb 033 and Liquiritigenin, as well as the pure ER antagonist ICI 182,780, were purchased from Tocris Bioscience (Bristol, United Kingdom).
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