Potassium chloride (kcl)

Acetic acid, disodium hydrogen phosphate, nicotinamide adenine dinucleotide reduced form (NADH), potassium chloride, phenazinemethosulphate, nitro-blue tetrazolium, disodium phosphate and sodium dihydrogen phosphates were procured from Sisco Research Laboratories (Mumbai, India). Reagents for Bradford assay were purchased from GeNei (Bengaluru, India). Hydrogen peroxide and potassium-dichromate were purchased from Merck (Darmstadt, Germany). Sodium dodecyl sulphate (SDS), thiobarbituric acid (TBA), trireagent, and zinc sulphate were procured from Sigma-Aldrich (St. Louis, MO, USA). Rabbit polyclonal to NMDAR2A (ab16646) antibody were procured from Abcam (place), mouse monoclonal anti-β-actin (sc47778) purchased from Santacruz (USA), biotinylated Goat polyclonal to Rabbit IgG and anti-mouse secondary antibodies, anti-goat AP conjugated and bovine anti-rabbit (AP) conjugated secondary antibodies were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA). DABCO, anti-fading agent was purchased from Fluka analytical (Shanghai, China). Secondary antibody, GT × Rb IgG-Biotin conjugated (62111028001A) and GT × Ms IgG Biotin-conjugated (cat#105261) (GeNei, Bengaluru, India). Streptavidin-peroxidase, normal goat serum and DAB system was procured from Genei Pvt. India Ltd. Polyvinylidene difloride (PVDF) membranes were procured from Merck Millipore (USA).

CagA antigen and antibodies were procured from the Max von Pettenkofer Institute of Hygiene and Medical Microbiology, Ludwig Maximilian University, Munich, Germany and stored at −20 °C till further use. The monomer, cross-linker, initiator, and methanol were purchased from Sigma-Aldrich, India, and were used without any further purification. Other chemicals, including K₃[Fe(CN)₆], K₄[Fe(CN)₆], and KCl, were purchased from Sisco Research Laboratory (SRL), Mumbai, India. All other chemicals were of analytical reagent (AR) grade and all aqueous solutions were prepared in double distilled water throughout the experiments. Cyclic voltammetric (CV) and electrochemical impedance studies (EIS) were performed using Bio-Logic Science Instruments SP-200 with EC-Lab^® software with an impedance analysis module. The working electrode was the screen-printed carbon electrode (SPE). Scanning electron microscopy (SEM) and Brunauer–Emmett–Teller (BET) were used to study the surface morphology and surface area of synthesized MIP with and without template CagA. The Material Studio version 2018r1 software package (Accelrys Inc., San Diego, CA, USA) [28 ] was used to conduct computational simulations.