Phenylmethylsulfonyl fluoride, sodium vanadate, bovine serum albumin, dithiothreitol (DTT), poly (Glu, Tyr), fluorescein isothiocyanate, holo-Transferrin, and buffer components were purchased from Sigma-Aldrich, St. Louis, MO, USA. Horseradish peroxidase linked anti-rabbit and anti-mouse secondary antibodies were from Cytiva, Marlborough, MA, USA. SuperSignal West Femto Chemiluminescent Substrate and Pierce ECL Western Blotting Substrate were purchased from Thermo Scientific, Waltham, MA, USA. Nickel-nitriloacetic acid (Ni-NTA) resin was from Qiagen, Germantown, MD, USA. The following antibodies and resin were obtained from MilliporeSigma, St. Louis, MO, USA: anti-phosphotyrosine clone 4G10 (05-321), anti-phospho-Ack1 (pY284) (09-142), anti-γ-Tubulin (T6557), horseradish peroxidase–linked anti-FLAG produced in mouse (F1804), and EZview red anti-FLAG M2 affinity resin. Anti-Mer antibody for Western blotting was from R&D Systems, Minneapolis, MN, USA (AF891). Anti-Mer antibody for immunoprecipitation was from abcam, Cambridge, UK (ab184086).
Anti phospho ack1 py284 09 142
Manufactured by Merck Group
Sourced in United States
Anti-phospho-Ack1 (pY284) (09-142) is an antibody used for the detection of Ack1 protein phosphorylated at tyrosine 284 in various applications, such as Western blotting and immunohistochemistry. It is a research-use-only product and not intended for diagnostic or therapeutic purposes.
Automatically generated - may contain errors
Lab products found in correlation
Sodium vanadate, by Merck Group (2 mentions)
Buffer components, by Merck Group (2 mentions)
Horseradish peroxidase linked anti rabbit and anti mouse secondary antibodies, by Cytiva (2 mentions)
Nickel nitriloacetic acid ni nta resin, by Qiagen (2 mentions)
Anti mer antibody for western blotting, by R&D Systems (2 mentions)
Anti mer antibody, by Abcam (2 mentions)
Holo transferrin, by Merck Group (2 mentions)
Supersignal west femto chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)
Pierce ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Fluorescein isothiocyanate (fitc), by Merck Group (2 mentions)
Phenylmethylsulfonyl fluoride, by Merck Group (2 mentions)
Anti phosphotyrosine clone 4g10 05 321, by Merck Group (2 mentions)
Anti γ tubulin t6557, by Merck Group (2 mentions)
Ezview red anti flag m2 affinity resin, by Merck Group (2 mentions)
2 protocols using anti phospho ack1 py284 09 142
1
Protein Purification and Immunoblotting Protocol
2
Protein Purification and Immunoblotting Protocol
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Phenylmethylsulfonyl fluoride, sodium vanadate, bovine serum albumin, dithiothreitol (DTT), poly (Glu, Tyr), fluorescein isothiocyanate, holo-Transferrin, and buffer components were purchased from Sigma-Aldrich, St. Louis, MO, USA. Horseradish peroxidase linked anti-rabbit and anti-mouse secondary antibodies were from Cytiva, Marlborough, MA, USA. SuperSignal West Femto Chemiluminescent Substrate and Pierce ECL Western Blotting Substrate were purchased from Thermo Scientific, Waltham, MA, USA. Nickel-nitriloacetic acid (Ni-NTA) resin was from Qiagen, Germantown, MD, USA. The following antibodies and resin were obtained from MilliporeSigma, St. Louis, MO, USA: anti-phosphotyrosine clone 4G10 (05-321), anti-phospho-Ack1 (pY284) (09-142), anti-γ-Tubulin (T6557), horseradish peroxidase–linked anti-FLAG produced in mouse (F1804), and EZview red anti-FLAG M2 affinity resin. Anti-Mer antibody for Western blotting was from R&D Systems, Minneapolis, MN, USA (AF891). Anti-Mer antibody for immunoprecipitation was from abcam, Cambridge, UK (ab184086).
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