Mortierella alpina atcc 32222

D-[U-¹³C]glucose (99% atom ¹³C) was purchased from Cambridge Isotope Laboratories (Andover, MA, USA). D-[U-¹⁴C]glucose (300–360 Ci/mol, 1mCi/mL) was purchased from American Radiolabeled Chemicals (Saint Louis, MO, USA). Unlabeled ARA was purchased from Nu-Check Prep Inc. (Elysian, MN). d₈-ARA was purchased from Cayman Chemical Company (Ann Arbor, MI). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA). Mortierella alpina (ATCC 32222) was purchased from the American Type Culture Collection (Manassas, VA, USA). Glassware and pipette tips were sterilized by autoclave. All manipulations were performed in a biosafety hood using sterile technique.