Penicillin streptomycin antibiotic solution

RAW 264.7 cells (ATCC^® TIB-71™), fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM), trypsin-EDTA solution (0.25% trypsin/0.53 mM EDTA in Hanks’s balanced salt solution (HBSS) without calcium or magnesium), and penicillin/streptomycin antibiotic solution were supplied by American Type Culture Collection (ATCC, Manassas, VA, USA). Centrifuge tubes equipped with 3 kDa molecular weight cut-off filters and water, methanol, far-UV acetonitrile, and chloroform were purchased from VWR International (West Chester, PA, USA). A C-Chip disposable hemocytometer was obtained from Bulldog Bio, Inc. (Portsmouth, NH, USA). HFIP-treated amyloid-peptide (1-42) was obtained from Bachem Distribution Services GmbH (Weil am Rhein, Germany). Sylgard 184 polydimethylsiloxane (PDMS) prepolymer and curing agent, used for the preparation of the hybrid microfluidic devices, were purchased from Ellsworth Adhesives (Germantown, WI, USA). Tentagel M NH₂ microspheres were obtained from Rapp Polymere GmbH (Tübingen, Germany). Rabbit IgG anti- 2,4-dinitrophenol (DNP) antibody (SP-0603-1) was purchased from Vector Laboratories Inc. (Burlingame, CA, USA). All the remaining materials, unless specified otherwise, were supplied by Sigma-Aldrich Corporate (St. Louis, MO, USA) or Thermo Fisher Scientific Inc. (Pittsburgh, PA, USA).